Title: The association of IL-17A polymorphisms with IL-17A serum levels and risk of ischemic stroke
Abstract: // Hua-Tuo Huang 1, * , Yu-Lan Lu 1, * , Rong Wang 1 , Hai-Mei Qin 1 , Chun-Fang Wang 1 , Jun-Li Wang 1 , Yang Xiang 1 , Jing Guo 3 , Yan Lan 3 and Ye-Sheng Wei 1, 2 1 Department of Clinical Laboratory, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, Guangxi, China 2 Youjiang Medical University for Nationalities, Baise 533000, Guangxi, China 3 Department of Dermatology, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, Guangxi, China * These authors have contributed equally to this work Correspondence to: Ye-Sheng Wei, email: [email protected] Yan Lan, email: [email protected] Keywords: IL-17A, genetic, promoter, polymorphisms, ischemic stroke Received: May 17, 2017 Accepted: September 05, 2017 Published: October 05, 2017 ABSTRACT The aim of our study was to investigate the association of interleukin-17A (IL-17A) polymorphisms with IL-17A serum levels and risk of ischemic stroke (IS) in a Chinese population. 392 IS patients and 443 controls were included in this study. The polymorphisms of IL-17A gene were determined by Snapshot SNP genotyping assay and DNA sequencing. Serum IL-17A levels were measured by enzyme-linked immunosorbent assay (ELISA). We found that the G allele, GA and GG genotypes, and GA/GG vs. AA model of rs2275913 polymorphism were associated with increased risk of IS even after adjusted by clinical characters such as age, gender and diabetes (G vs. A: OR=1.27, 95% CI, 1.05~1.54, P =0.014; GA vs. AA: OR=1.72, 95% CI, 1.05~2.81, P =0.032; GG vs. AA: OR=1.99, 95% CI, 1.08~3.67, P =0.028; GA/GG vs. AA: OR=1.78, 95% CI, 1.11~2.86, P =0.017). Serum IL-17A levels were increased in IS patients compared with controls ( P <0.01). Individuals carrying rs2275913 GA or GG genotype present higher serum IL-17A levels compared with the rs2275913AA genotype in the IS group ( P <0.01). In conclusion, this is the first study reporting the rs2275913 polymorphism as a risk factor for IS, which may be partly explained by influencing the levels of IL-17A cytokine.