Title: INHIBITION OF GROWTH AND METASTASIS OF HUMAN GASTRIC CANCER IMPLANTED IN NUDE MICE BY D-LIMONENE
Abstract: Objective To study the effects and mechanism of D-limonene on the growth and metastasis of gastric cancer in vivo. Methods Metastatic model simulating human gastric cancer was established by orthotopic implantation of histologically intact human tumor tissue into gastric wall of nude mice.D-limonene was orally administered at dose of 15ml/kg every other day for seven weeks. Eight weeks after implantation, tumor weight, MVD, apoptotic index(AI), electron microscopy was used to observe the super structure changes of tumor;variation of ultra-structure, and the presence of metastasis were evaluated respectively after the mice were sacrificed. Results The growth and metastasis of human gastric cancer implanted in nude mice were significantly inhibited in D-limonene group and combined group than that in control group by tumors in weight, MVD and VEGF, the AI and inhibition rate were increased significantly(P0.05). The incidences of peritoneal metastasis and liver metastasis also decreased significantly in D-limonene group and combined group than that in control group, 5-FU group(P0.05).Conclusion D-limonene can induce apoptosis in gastric cancer by inhibiting tumor angiogenesis and has strong inhibitory effect both on tumor growth and metastasis of human gastric cancer implanted in nude mice.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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Cited By Count: 17
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