Title: The relationship between CXCL12/CXCR4 biological axis and neck lymphatic metastasis hi head and neck squamous carcinoma
Abstract: Objective To analyze the relationship between the expression of CXCR4 and CXCL12 in head and neck squamous carcinoma(HNSCC) and clinicopathological features of HNSCC, and explore the effect of CXCR4/CXCL12 biological axis on lymph node metastasis in HNSCC. Methods The clinicopathological data of 65 patients with HNSCC undergoing surgical resection were collected and reviewed. Fifteen cases of normal mucosa and benign lesion served as controls. The differential expression of CXCR4 and CXCL12 in different groups was detected by using immunohistochemical staining and reverse transcrip-tion-polymerase chain reaction(RT-PCR) to identify their relationship with lymph node metastasis. Results The expression of CXCR4 mRNA was 0.406 ± 0. 044, 0.464 ± 0. 068 and 0. 900 ± 0. 108 in lymp metastasis, non-lymph metastasis and control groups respectively. There was significant difference in the CXCR4 mRNA expression between HNSCC group and control group (P 0.05 ). The statistical analysis revealed that the expression rate of CSCR4 protein was 71.4% (25/35), 33. 3% (10/30) and 13.3% (2/15) in lymph metastasis, non-metastasis and control groups respectively. Over-expression of CXCR4 was significantly correlated with clinical stage, depth of invasion and lymph node metastasis (P<0.01). There was no significant difference in the CXCL12 expression among the groups. Conclusion Overexpression of CXCR4 was related to lymph node metastasis and classification in HNSCC. CXCL12/CXCR4 biological axis might promote orientation metastasis to cervical lymph node in HNSCC.
Key words:
Head and neck squamous carcinoma; Lymphatic metastasis
Publication Year: 2010
Publication Date: 2010-07-08
Language: en
Type: article
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