Title: Isolation of antagonistic fungi and evaluation of antifungal activity of the separated metabolite against the red rot of sugarcane pathogen
Abstract: Antagonistic potential of six rhizosphere soil fungi viz., Trichoderma harzianum, T. viride, Aspergillus flavus, A.
fumigatus, A. niger, and A. sulphureus were tested against the red rot of sugarcane pathogen Colletotrichum
falcatum. Out of the seven soil fungi tested, Trichoderma harzianum was found to be most effective in controlling the
growth of Colletotrichum falcatum based on the colony interactions and effects of volatile and non-volatile
metabolites. When HPLC fractionation of the mycelia extract of T. harzianum, T. viride, Aspergillus flavus, A.
fumigatus, A. niger, and A. sulphureus were tested against the red rot of sugarcane pathogen C. falcatum. The T.
harzianum peak at showed RT 4.893 maximum antifungal activity with the inhibition zone of 0.9 mm followed
by Trichoderma viride(0.7mm), A. fumigatus (0.5 mm), A. niger (0.5mm), A. flavus ( 0.4 mm), and A. sulphureus (0.3mm). This peak may be responsible for the antifungal activity of this fraction. The fungus isolated in the present
study may be exploited commercially to biocontrol this disease. Further studies on the determination of the actual
biochemical compound responsible for antagonistic effect through FT-IR and GC-MS analysis is under way.
Publication Year: 2016
Publication Date: 2016-01-01
Language: en
Type: article
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Cited By Count: 4
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