Title: In vitro propagation of Calamus nagbettai : An endangered plant
Abstract: An efficient protocol for plantlet regeneration from collar region of seedlings and embryo in economically important plant Calamus nagbettai (Arecaceae), endemic to Western Ghats of Karnataka has been developed. For callus induction, the explants were cultured on MS medium augmented with NAA (3mg/l), IBA (4mg/l) and 2, 4-D (8mg/l) separately. In 2,4-D augmented media the frequency of caulogenesis observed was 94%. The study clearly showed that, 2,4-D is highly effective to induce callus. The calli were subcultured on MS medium with various concentrations of cytokinins for shoot formation. The medium supplemented with BAP (3mg/l) showed good response for shoot formation (82.4%). The In Vitro produced shoots were cultured on MS medium fortified with various concentrations of GA3 for further elongation. The medium supplemented with1.0 mg/l GA3 was found suitable for elongation of microshoots. The shoots were rooting best in ½ MS medium containing IBA (1mg/l) and NAA (0.5mg/l) separately. The rooted plantlets were transferred to the field after acclimatization. Key words: Weatern Ghats, Calamus nagbettai, callus, organogenesis, multiple shoots.
Publication Year: 2017
Publication Date: 2017-03-18
Language: en
Type: article
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Cited By Count: 3
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