Title: S0230103 Disruption of capillary-like structure and increased endothelial permeability by exposure to impulsive pressure
Abstract: Intracranial pressure changes during head impact cause brain injuries such as vasogenic edema and cerebral contusion. The purpose of this research is better understanding of the effect of impulsive pressure during head impact on the disruption of blood-brain barrier (BBB) that forms a physical barrier to filter blood-borne substances between the blood and the brain parenchyma. Firstly, changes in the capillary-like network formed by endothelial cells were observed using an inverted microscope. The capillary-like structure was broken down after 1 h following impulsive pressure, and the capillary network gradually disappeared until 24 h after loading. Then, following impulsive pressure loading, transendothelial electrical resistance (TEER) was measured using the in vitro BBB models consisting of cultured endothelial cells, pericytes and astrocytes from rat brain capillaries. Lower TEER indicates the disruption of endothelial integrity and the formation of intercellular gap. In the result, TEER decreased immediately after loading and the decrease in TEER was larger in the co-culture model with endothelial cells and astrocytes than in the endothelial monolayer model or the co-culture model with endothelial cells and pericytes. These results suggest that the impulsive pressure during head impact impair endothelial barrier function by the disruption of the integrity of endothelial cell-cell junctions, and the degree of increase in endothelial permeability depends on injured cells that compose BBB.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
Indexed In: ['crossref']
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