Title: Effect of rewatering on activity of protect enzymes and membrane peroxidation in Robinia pseudoacacia seedling.
Abstract: 【Objective】 Physiological changes during water stress and rewatering in Robinia pseudoacacia seedling leaves were studied to investigate the compensation effect that was woken by water stress.【Method】 A potted experiment was conducted in 30 days and with 5 different soil relative water content(SRW) levels,which were 100.00%,87.84%,70.00%,52.16% and 40.00%.The activities of superoxide dismutase(SOD) and peroxidase(POD),the content of superoxide hydronium(·O2-)and malondialdehyde(MDA) in leaves of one-year-old R.pseudoacacia were tested under different water stress levels and 2,24,48,72 h later after rewatering.【Result】 During the same period of water stress,the content of superoxide hydronium(·O2-) and malondialdehyde(MDA) rose significantly compared with control treatment.Meanwhile the activities of superoxide dismutase(SOD) and peroxidase(POD) were boosting and could still maintain at a high level of active oxygen eliminating.The activities of superoxide dismutase(SOD) and peroxidase(POD) were lower in SRW of 40.00% than that of 52.16%,but still higher than that of control treatment.The activities of superoxide dismutase(SOD) and peroxidase(POD) soared,however the content of superoxide hydronium(·O2-) and malondialdehyde(MDA) declined quickly 2 h after rewatering.The active oxygen eliminating ability of protect enzymes in leaves of R.pseudoacacia seedling was strengthened by water stress compared with control treatment.【Conclusion】 The antioxidation ability could be strengthened by proper water stress in R.pseudoacacia seedling.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 1
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