Title: Production of intrinsically unstructured proteins involved in formation of enamel matrix
Abstract:Tooth enamel is the hardest tissue of the body of vertebrates. It is formed by 98 % of hydroxylapatit mineral organized in so-called rods surrounded by interrod substance. Enamel is formed by process ...Tooth enamel is the hardest tissue of the body of vertebrates. It is formed by 98 % of hydroxylapatit mineral organized in so-called rods surrounded by interrod substance. Enamel is formed by process called biomineralization as a secreted product of ameloblasts. During amelogenesis these special cells produce a great amount of proteins, especially amelogenin, ameloblastin, enamelin, tuftelin and many others. These proteins are used as scaffolds for hydroxylapatite crystals and they are necessary for further nucleation, growth and formation of these crystals. Moreover they have many other biologically important functions. The aim of this thesis was to prepare wild type ameloblastin and its mutant without the part encoded by exon 5 in expression system of E. coli and to optimize their purification protocol. Next aim was to test potential antimicrobial behaviour of these proteins.Read More
Publication Year: 2016
Publication Date: 2016-01-01
Language: en
Type: article
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