Title: 3P202 Analysis of interaction of the DnaJ family protein SflA, that is involved in regulation of flagellation in Vibrio alginolyticus(Cell biology,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))
Abstract:QBiC, RIKEN) Leptospira have intracellular flagella that rotate within the periplasmic space.Each flagellar filament is linked to a flagellar motor.Although the bacterial flagellar motor is powered by...QBiC, RIKEN) Leptospira have intracellular flagella that rotate within the periplasmic space.Each flagellar filament is linked to a flagellar motor.Although the bacterial flagellar motor is powered by the electrochemical potential gradient of specific ions across the cytoplasmic membrane, coupling ions driving the Leptospira motor has not been investigated.We tested effects of a sodium channel inhibitor, EIPA, and a protonophore, CCCP, on the motility of saprophytic Leptospira biflexa, showing that the motility is completely inhibited by EIPA but not by CCCP.We also found that the motility is significantly enhanced with concentrations of NaCl added to media.These results suggest that the flagellar motor of Leptospira can utilize Na + as a coupling ion for torque generation.Read More