Title: Interleukin-6 increases resistance to anti-androgen therapy via TIF2
Abstract:AACR Annual Meeting-- Apr 12-16, 2008; San Diego, CA
697
Interleukin-6 (IL-6) is a pleiotropic cytokine that plays a critical role in the development and progression of prostate cancer. The levels ...AACR Annual Meeting-- Apr 12-16, 2008; San Diego, CA
697
Interleukin-6 (IL-6) is a pleiotropic cytokine that plays a critical role in the development and progression of prostate cancer. The levels of IL-6 in serum are significantly elevated in many men with advanced, hormone-refractory prostate cancer. Numerous studies have suggested the role of IL-6 in the growth and androgen responsiveness of prostate cancer cells in vitro and in vivo . To study the role of IL-6 in prostate cancer cells in response to androgen deprivation therapy, series of lower and higher passages of LNCaP cell sublines were generated by a long-term exposure of LNCaP cells in IL-6 containing culture media. The higher passages of LNCaP cells treated with IL-6 are more resistant to bicalutamide treatment compared to parental LNCaP cells. To understand the mechanisms of IL-6 mediated resistance to bicalutamide treatment, we compared the expression of coactivator TIF2 expression in IL-6 treated LNCaP cells and parental LNCaP cells. The levels of TIF2 in IL-6 treated LNCaP cells were found significantly higher than the parental LNCaP cells. Down regulation of TIF2 expression via siRNA in IL-6 treated LNCaP cells decreased resistance of these cells to bicalutamide treatment; while over expression TIF2 in the parental LNCaP cells increased resistance of these cells to bicalutamide treatment. Collectively, these results suggest that overexpression of IL-6 increases prostate cancer cells resistance to bicalutamide treatment via overexpression of TIF2.Read More
Publication Year: 2008
Publication Date: 2008-05-01
Language: en
Type: article
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Cited By Count: 1
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