Title: Using SCAR molecular marker to detect resistance genes to Fusarium oxysporum f. sp. ciceris in chickpea cultivars and lines
Abstract: The occurrence of fusarium wilt disease is reported from 32 countries around the world. It is
estimated that this disease results in 10-90% crop yield loss annually. It is difficult to control this disease
because of the ability of the pathogen to survive in soil for several years, even without the host. The most
effective method for controlling fusarium wilt is the cultivation of resistant chickpea cultivars. In this
experiment, SCAR molecular marker was used to detect chickpea genotypes resistant to the disease. The
DNA of 42 chickpea genotypes was extracted by CTAB method. After that, polymerase chain reaction was
conducted by CS-27 and OPM-20 molecular markers. Results showed that 41 genotypes out of the 42 tested
genotypes were sensitive to all five races of the pathogen. The only resistant genotype was Flip 06-152c which
was resistant to the five races. Finally, the pathogenesis test confirms all results obtained in the genetic
studies.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 2
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