Real-time Reverse Transcription Polymerase Chain Reaction Using Total RNA Extracted from Nasopharyngeal Aspirates for Detection of Pneumococcal Carriage in Children

Name: Work Video:
Duration: 3 min 30 s
Young Kwang Kim; Kyoung Hoon Lee; Ki Wook Yun; Mi Kyung Lee; In Seok Lim
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'abstract_inverted_index': {'목적:': [0], '폐렴구균은': [1], '주요': [2, 200], '비인두': [3, 21, 64, 178], '상재균으로,': [4], '주위': [5], '조직을': [6], '침범하여': [7], '침습성': [8], '감염을': [9], '일으킬': [10], '수': [11, 30, 208], '있어': [12], '보균': [13], '율에': [14], '대한': [15], '감시가': [16], '중요하다.': [17], '본': [18, 176], '연구에서는': [19], '임상에서': [20], '흡인물로부터': [22], '추출하고': [23], '남은': [24], 'RNA를': [25, 95], '이용': [26], '하여': [27], '폐렴구균을': [28], '확인할': [29], '있는': [31, 209], '실시간': [32], '중합효소': [33], '연쇄반응(real-time': [34], 'reverse': [35], 'transcription': [36], 'poly-merase': [37], 'chain': [38], 'reaction': [39], '[RT-PCR])법을': [40], '구축하고,': [41], '보균율': [42, 186, 202], '측정에': [43, 187], '있어서의': [44, 188], '정확성과': [45], '이점을': [46], '확인하고자': [47], '하였다.': [48], '방법:': [49], '2014년': [50], '9월부터': [51], '10월까지': [52], '중앙대학교병원에': [53], '입원하여': [54], '호흡기': [55], '바이러스': [56], 'RT-PCR': [57, 149, 170, 190, 197], '검사를': [58], '시행': [59], '받은': [60], '18세': [61], '이하의': [62], '소아들로부터': [63], '흡인물을': [65], '채취하였다.': [66], '먼저': [67], '배양법과': [68, 89], 'genomic': [69], 'DNA': [70], '(gDNA)': [71], '를': [72, 86], '이용한': [73, 91, 96], 'real-time': [74, 92, 97, 108, 125, 137, 148, 157, 169, 182, 189], 'PCR을': [75], '시행하여': [76], '폐렴구균': [77, 185], '검출률의': [78], '정확성을': [79], '확인하였다.': [80], '이': [81], '중': [82, 134], '처음': [83], '20개의': [84, 145], '검체': [85, 133], '이용하여,': [87], '고전적인': [88], 'gDNA를': [90], 'PCR,': [93], '그리고': [94], 'RT-PCR법을': [98], '시행하고': [99, 163], '이를': [100], '비교': [101], '분석하였다.': [102], '결과:': [103], '총': [104, 167, 172], '157개의': [105], '검체에서': [106, 146], '시행한': [107, 147], 'PCR': [109], '검사는': [110, 150], '기존의': [111], '배양검사와': [112], '일치율이': [113], '0.922': [114], '(P<0.01,': [115], 'Fisher': [116], 'exact': [117], 'test)로': [118], '매우': [119], '높았다.': [120], '배양검사에서': [121], '음성인': [122], '133개의': [123], '검체는': [124, 136, 141], 'PCR에서도': [126, 138], '모두': [127], '음성을': [128], '보였다.': [129, 144], '24개의': [130], '배양': [131], '양성': [132], '21개의': [135], '양성이었지만,': [139], '나머지': [140], '음성': [142], '결과를': [143, 164], '1개': [151], '검체를': [152], '제외': [153], '하고': [154], '배양법': [155], '및': [156, 193], 'PCR과': [158], '결과가': [159], '일치하였다.': [160], '한편,': [161], '배양법을': [162], '확인하기까지': [165], '는': [166], '26.5시간,': [168], '검사에는': [171], '4.5시간이': [173], '소요되었다.': [174], '결론:': [175], '연구는': [177], '집락균': [179], '확인을': [180], '위한': [181], 'RT-PCR법의': [183], '확립과,': [184], '검사의': [191], '정확성': [192], '편의성을': [194], '보여주었다.': [195], 'Real-time': [196], '검사법': [198], '은': [199], '세균들의': [201], '연구에': [203], '있어서': [204], '시간과': [205], '노력을': [206], '줄일': [207], '좋은': [210], '방법이며,': [211], '폐렴구균의': [212], '역학': [213], '자료': [214], '수집에': [215], '큰': [216], '도움이': [217], '될': [218], '것으로': [219], '기대한다.': [220]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2598733973', 'counts_by_year': [{'year': 2022, 'cited_by_count': 1}], 'updated_date': '2024-12-10T07:53:40.451154', 'created_date': '2017-04-07'}
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