Title: Somatic Embryogenesis and Plant Regeneration from Cell Suspension Culture of Gymnema sylvestre (Retz) R. Br. Ex Roemer & Schultes
Abstract:Abstract We have optimized a system for the somatic embryogenesis via embryogenic suspension cultures of Gymnema sylvestre. Callus cultures were induced on MS medium with growth regulators (2,4 -D 0....Abstract We have optimized a system for the somatic embryogenesis via embryogenic suspension cultures of Gymnema sylvestre. Callus cultures were induced on MS medium with growth regulators (2,4 -D 0.5 mg/l (or) NAA 1.0 mg/l) and 10% coconut water. They were transferred into MS liquid medium containing NAA 1.0 mg/l, BA 1.0 mg/l, 3.0% sucrose (w/v), 10% coconut water, citric acid 1 mg/l and glutamine 10 mg/l for somatic embryogenesis from callus. Globular, heart, torpedo and cotyledonary stage of embryos were observed in suspension cultures after 8 weeks. The maturation embryos were significantly affected by growth regulators and photoperiod. Five to seven percent of embryos formed plantlets on semisolid medium containing basal MS medium with B5 vitamin, 3.0% sucrose and 0.8% agar (w/v). All plantlets established in the field exhibited morphological characters similar to those of the mother plant.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 7
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