Title: Determination of PQQ by Capillary Zone Electrophoresis
Abstract: A new method for the determination of pyrroloquinoline quinone
by capillary zone electrophoresis has been developed.
Separation conditions have been optimised with the respect to
different parameters including pH and ionic strength of the
background electrolyte, separation voltage and temperature of
the capillary. A buffer consisting of 50 mM b-alanine - HCl pH
3.0 was found to be the most suitable electrolyte for this
separation. An applied voltage of 25 kV (negative polarity) and
a temperature of 25 oC gave the best analysis of
pyrroloquinoline quinone. The linear detection range for
concentration versus peak area for the assay is from 5 to 500
mM (correlation coefficient 0.9998) with a detection limit of
0.1- 0.2 mM. The inter-day reproducibility of the peak area was
2.5 % and the inter-day reproducibility of the migration time
was below 0.18 %.
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
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