Title: CYTOTOXIC AND APOPTOSIS-INDUCING EFFECT OF LUTEOLIN ISOLATED FROM FERONIA LIMONIA ON HEPG2 CELLS
Abstract: Hepatocellular carcinoma (HCC) is one of the most commonly detected cancers. Traditional medicines have long been used for the treatment of this cancer. The objective of this study was to screen the anticancer activities in human hepatocellular carcinoma (HepG2) cell line of the isolated luteolin from Feronia limonia. Cytotoxic and apoptotic activities of luteolin were performed on HepG2 cell line by MTT assay, ethidium bromide (EtBr) staining & DNA fragmentation technique respectively. Vincristine was used as the standard drug. The results showed that the luteolin at 100 μg/ml has the highest cytotoxic activity with the IC50 (concentration that inhibits cell growth by 50%) value with 48.23 % inhibition. The compound exhibited the highest apoptotic induction at the IC50 concentration. This study has confirmed the potential anti-cancer activity of luteolin from Feronia limonia which can be further developed as a novel drug.