Title: Effect of cryoprotectants on the morphology of fish spermatozoa.
Abstract: A study was carried out to identify the best cryoprotectant for
the cryopreservation of spermatozoa of Liza parsia, a brackish / marine fish of great culture potential. Various cryoprotectants alone and in combinations, at different concentrations have been tried. Morphology of spermatozoa before and after cryopreservation studied by transmission electron microscopy (TEM). A combination of 5% DMSO and 5% glycerol in V2E extender accorded better protection than 10% DMSO alone. The toxic effects of high concentrations of DMSO can be minimised by combining with glycerol.
Publication Year: 2002
Publication Date: 2002-07-01
Language: en
Type: dissertation
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