Title: Bile acids potentiate proton‐activated currents in <i>Xenopus laevis</i> oocytes expressing human acid‐sensing ion channel (<scp>ASIC</scp>1a)
Abstract: Abstract Acid‐sensing ion channels ( ASIC s) are nonvoltage‐gated sodium channels transiently activated by extracellular protons and belong to the epithelial sodium channel ( EN aC)/Degenerin ( DEG ) family of ion channels. Bile acids have been shown to activate two members of this family, the bile acid‐sensitive ion channel ( BASIC ) and EN aC. To investigate whether bile acids also modulate ASIC function, human ASIC 1a was heterologously expressed in Xenopus laevis oocytes. Exposing oocytes to tauro‐conjugated cholic (t‐ CA ), deoxycholic (t‐ DCA ), and chenodeoxycholic (t‐ CDCA ) acid at pH 7.4 did not activate ASIC 1a‐mediated whole‐cell currents. However, in ASIC 1a expressing oocytes the whole‐cell currents elicited by pH 5.5 were significantly increased in the presence of these bile acids. Single‐channel recordings in outside‐out patches confirmed that t‐ DCA enhanced the stimulatory effect of pH 5.5 on ASIC 1a channel activity. Interestingly, t‐ DCA reduced single‐channel current amplitude by ~15% which suggests an interaction of t‐ DCA with a region close to the channel pore. Molecular docking predicted binding of bile acids to the pore region near the degenerin site (G433) in the open conformation of the channel. Site‐directed mutagenesis demonstrated that the amino acid residue G433 is critically involved in the potentiating effect of bile acids on ASIC 1a activation by protons.