Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2586333749', 'doi': 'https://doi.org/10.21769/bioprotoc.2126', 'title': 'Polysome Fractionation to Analyze mRNA Distribution Profiles', 'display_name': 'Polysome Fractionation to Analyze mRNA Distribution Profiles', 'publication_year': 2017, 'publication_date': '2017-01-01', 'ids': {'openalex': 'https://openalex.org/W2586333749', 'doi': 'https://doi.org/10.21769/bioprotoc.2126', 'mag': '2586333749', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/28516123', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/5431591'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.21769/bioprotoc.2126', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4210179406', 'display_name': 'BIO-PROTOCOL', 'issn_l': '2331-8325', 'issn': ['2331-8325'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320252', 'host_organization_name': 'American Academy of Arts and Sciences', 'host_organization_lineage': ['https://openalex.org/P4310320252'], 'host_organization_lineage_names': ['American Academy of Arts and Sciences'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'green', 'oa_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431591', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5006877808', 'display_name': 'Amaresh C. Panda', 'orcid': 'https://orcid.org/0000-0003-3189-8995'}, 'institutions': [{'id': 'https://openalex.org/I4210136305', 'display_name': 'Institute on Aging', 'ror': 'https://ror.org/0390vdb22', 'country_code': 'US', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I4210136305']}, {'id': 'https://openalex.org/I4210136897', 'display_name': 'National Institute on Aging', 'ror': 'https://ror.org/049v75w11', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210136897']}, {'id': 'https://openalex.org/I1299303238', 'display_name': 'National Institutes of Health', 'ror': 'https://ror.org/01cwqze88', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Amaresh C Panda', 'raw_affiliation_strings': ['Laboratory of Genetics and Genomics, National Institute on Aging, National Institutes of Health, Biomedical Research Center, Baltimore, USA.'], 'affiliations': [{'raw_affiliation_string': 'Laboratory of Genetics and Genomics, National Institute on Aging, National Institutes of Health, Biomedical Research Center, Baltimore, USA.', 'institution_ids': ['https://openalex.org/I4210136305', 'https://openalex.org/I4210136897', 'https://openalex.org/I1299303238']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5006152899', 'display_name': 'Jennifer L. Martindale', 'orcid': 'https://orcid.org/0000-0002-3234-6861'}, 'institutions': [{'id': 'https://openalex.org/I4210136305', 'display_name': 'Institute on Aging', 'ror': 'https://ror.org/0390vdb22', 'country_code': 'US', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I4210136305']}, {'id': 'https://openalex.org/I4210136897', 'display_name': 'National Institute on Aging', 'ror': 'https://ror.org/049v75w11', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210136897']}, {'id': 'https://openalex.org/I1299303238', 'display_name': 'National Institutes of Health', 'ror': 'https://ror.org/01cwqze88', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Jennifer L Martindale', 'raw_affiliation_strings': ['Laboratory of Genetics and Genomics, National Institute on Aging, National Institutes of Health, Biomedical Research Center, Baltimore, USA.'], 'affiliations': [{'raw_affiliation_string': 'Laboratory of Genetics and Genomics, National Institute on Aging, National Institutes of Health, Biomedical Research Center, Baltimore, USA.', 'institution_ids': ['https://openalex.org/I4210136305', 'https://openalex.org/I4210136897', 'https://openalex.org/I1299303238']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5044404083', 'display_name': 'Myriam Gorospe', 'orcid': 'https://orcid.org/0000-0001-5439-3434'}, 'institutions': [{'id': 'https://openalex.org/I4210136305', 'display_name': 'Institute on Aging', 'ror': 'https://ror.org/0390vdb22', 'country_code': 'US', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I4210136305']}, {'id': 'https://openalex.org/I4210136897', 'display_name': 'National Institute on Aging', 'ror': 'https://ror.org/049v75w11', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210136897']}, {'id': 'https://openalex.org/I1299303238', 'display_name': 'National Institutes of Health', 'ror': 'https://ror.org/01cwqze88', 'country_code': 'US', 'type': 'government', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Myriam Gorospe', 'raw_affiliation_strings': ['Laboratory of Genetics and Genomics, National Institute on Aging, National Institutes of Health, Biomedical Research Center, Baltimore, USA.'], 'affiliations': [{'raw_affiliation_string': 'Laboratory of Genetics and Genomics, National Institute on Aging, National Institutes of Health, Biomedical Research Center, Baltimore, USA.', 'institution_ids': ['https://openalex.org/I4210136305', 'https://openalex.org/I4210136897', 'https://openalex.org/I1299303238']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 3, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 0, 'currency': 'USD', 'value_usd': 0, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 4.277, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 127, 'citation_normalized_percentile': {'value': 0.999983, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 98, 'max': 99}, 'biblio': {'volume': '7', 'issue': '3', 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10521', 'display_name': 'RNA and protein synthesis mechanisms', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10521', 'display_name': 'RNA and protein synthesis mechanisms', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11482', 'display_name': 'RNA modifications and cancer', 'score': 0.9998, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10604', 'display_name': 'RNA Research and Splicing', 'score': 0.9998, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/polysome', 'display_name': 'Polysome', 'score': 0.96323025}, {'id': 'https://openalex.org/keywords/translational-regulation', 'display_name': 'Translational regulation', 'score': 0.5709893}, {'id': 'https://openalex.org/keywords/translational-efficiency', 'display_name': 'Translational efficiency', 'score': 0.48671415}, {'id': 'https://openalex.org/keywords/ribosome-profiling', 'display_name': 'Ribosome profiling', 'score': 0.41742292}], 'concepts': [{'id': 'https://openalex.org/C65666806', 'wikidata': 'https://www.wikidata.org/wiki/Q970614', 'display_name': 'Polysome', 'level': 5, 'score': 0.96323025}, {'id': 'https://openalex.org/C88478588', 'wikidata': 'https://www.wikidata.org/wiki/Q42244', 'display_name': 'Ribosome', 'level': 4, 'score': 0.7938617}, {'id': 'https://openalex.org/C105580179', 'wikidata': 'https://www.wikidata.org/wiki/Q188928', 'display_name': 'Messenger RNA', 'level': 3, 'score': 0.71768963}, {'id': 'https://openalex.org/C149364088', 'wikidata': 'https://www.wikidata.org/wiki/Q185917', 'display_name': 'Translation (biology)', 'level': 4, 'score': 0.70295936}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.66384137}, {'id': 'https://openalex.org/C67705224', 'wikidata': 'https://www.wikidata.org/wiki/Q11053', 'display_name': 'RNA', 'level': 3, 'score': 0.5753434}, {'id': 'https://openalex.org/C39685955', 'wikidata': 'https://www.wikidata.org/wiki/Q3455905', 'display_name': 'Translational regulation', 'level': 5, 'score': 0.5709893}, {'id': 'https://openalex.org/C3675279', 'wikidata': 'https://www.wikidata.org/wiki/Q211935', 'display_name': 'Protein biosynthesis', 'level': 2, 'score': 0.54455256}, {'id': 'https://openalex.org/C150194340', 'wikidata': 'https://www.wikidata.org/wiki/Q26972', 'display_name': 'Gene expression', 'level': 3, 'score': 0.5221658}, {'id': 'https://openalex.org/C162317418', 'wikidata': 'https://www.wikidata.org/wiki/Q252857', 'display_name': 'Transcriptome', 'level': 4, 'score': 0.52085215}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.49394637}, {'id': 'https://openalex.org/C2775992057', 'wikidata': 'https://www.wikidata.org/wiki/Q16956568', 'display_name': 'Translational efficiency', 'level': 5, 'score': 0.48671415}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.43298167}, {'id': 'https://openalex.org/C182325514', 'wikidata': 'https://www.wikidata.org/wiki/Q7322425', 'display_name': 'Ribosome profiling', 'level': 5, 'score': 0.41742292}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.357031}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.2824209}], 'mesh': [], 'locations_count': 4, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.21769/bioprotoc.2126', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4210179406', 'display_name': 'BIO-PROTOCOL', 'issn_l': '2331-8325', 'issn': ['2331-8325'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320252', 'host_organization_name': 'American Academy of Arts and Sciences', 'host_organization_lineage': ['https://openalex.org/P4310320252'], 'host_organization_lineage_names': ['American Academy of Arts and Sciences'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431591', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc5431591', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'acceptedVersion', 'is_accepted': True, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/28516123', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431591', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 5, 'referenced_works': ['https://openalex.org/W1980914060', 'https://openalex.org/W2054729735', 'https://openalex.org/W2122206493', 'https://openalex.org/W2128551987', 'https://openalex.org/W2301925216'], 'related_works': ['https://openalex.org/W4323351467', 'https://openalex.org/W4300980791', 'https://openalex.org/W4214696265', 'https://openalex.org/W2945902794', 'https://openalex.org/W2749718530', 'https://openalex.org/W2595978746', 'https://openalex.org/W2263061237', 'https://openalex.org/W2208711566', 'https://openalex.org/W2154495673', 'https://openalex.org/W1989588698'], 'abstract_inverted_index': {'Eukaryotic': [0], 'cells': [1], 'adapt': [2], 'to': [3, 105, 159, 173], 'changes': [4, 49], 'in': [5, 43, 50, 94], 'external': [6], 'or': [7, 168], 'internal': [8], 'signals': [9], 'by': [10, 61], 'precisely': [11], 'modulating': [12], 'the': [13, 27, 33, 36, 107, 120, 128, 186, 193, 196], 'expression': [14, 20, 52], 'of': [15, 21, 38, 57, 83, 109, 123, 130, 189, 195], 'specific': [16], 'gene': [17], 'products.': [18], 'The': [19, 54, 81], 'protein-coding': [22], 'genes': [23], 'is': [24, 40, 59, 90, 101], 'controlled': [25], 'at': [26, 178, 192], 'transcriptional': [28], 'and': [29, 65, 75, 137], 'post-transcriptional': [30], 'levels.': [31], 'Among': [32], 'latter': [34], 'steps,': [35], 'regulation': [37], 'translation': [39, 89, 190], 'particularly': [41], 'important': [42], 'cellular': [44], 'processes': [45], 'that': [46, 85, 124, 140, 156], 'require': [47], 'rapid': [48], 'protein': [51], 'patterns.': [53], 'translational': [55, 121], 'efficiency': [56], 'mRNAs': [58, 139, 148, 164], 'altered': [60], 'RNA-binding': [62], 'proteins': [63], '(RBPs)': [64], 'noncoding': [66], '(nc)RNAs': [67], 'such': [68], 'as': [69, 199, 201], 'microRNAs': [70], '(Panda': [71, 144], 'et': [72, 78, 145], 'al.,': [73, 79, 146], '2014a': [74], '2014b;': [76], 'Abdelmohsen': [77], '2014).': [80], 'impact': [82], 'factors': [84], 'regulate': [86], 'selective': [87], 'mRNA': [88], 'a': [91, 102, 112], 'critical': [92], 'question': [93], 'RNA': [95], 'biology.': [96], 'Polyribosome': [97], '(polysome)': [98], 'fractionation': [99, 183], 'analysis': [100, 184], 'powerful': [103], 'method': [104], 'assess': [106], 'association': [108], 'ribosomes': [110, 131, 152, 170], 'with': [111, 132, 150, 166], 'given': [113], 'mRNA.': [114], 'It': [115], 'provides': [116], 'valuable': [117], 'information': [118], 'about': [119], 'status': [122], 'mRNA,': [125], 'depending': [126], 'on': [127], 'number': [129], 'which': [133], 'they': [134], 'are': [135, 141, 157, 171], 'associated,': [136], 'identifies': [138], 'not': [142], 'translated': [143, 175], '2016).': [147], 'associated': [149, 165], 'many': [151], 'form': [153], 'large': [154], 'polysomes': [155], 'predicted': [158], 'be': [160, 174], 'actively': [161], 'translated,': [162], 'while': [163], 'few': [167], 'no': [169], 'expected': [172], 'poorly': [176], 'if': [177], 'all.': [179], 'In': [180], 'sum,': [181], 'polysome': [182], 'allows': [185], 'direct': [187], 'determination': [188], 'efficiencies': [191], 'level': [194], 'whole': [197], 'transcriptome': [198], 'well': [200], 'individual': [202], 'mRNAs.': [203]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2586333749', 'counts_by_year': [{'year': 2024, 'cited_by_count': 17}, {'year': 2023, 'cited_by_count': 24}, {'year': 2022, 'cited_by_count': 25}, {'year': 2021, 'cited_by_count': 18}, {'year': 2020, 'cited_by_count': 19}, {'year': 2019, 'cited_by_count': 11}, {'year': 2018, 'cited_by_count': 11}, {'year': 2017, 'cited_by_count': 1}], 'updated_date': '2024-12-11T13:13:37.890658', 'created_date': '2017-02-17'}