Publication Information

{'id': 'https://openalex.org/W2580906481', 'doi': 'https://doi.org/10.1021/acs.analchem.6b04997', 'title': 'Generic Hybrid Ligand Binding Assay Liquid Chromatography High-Resolution Mass Spectrometry-Based Workflow for Multiplexed Human Immunoglobulin G1 Quantification at the Intact Protein Level: Application to Preclinical Pharmacokinetic Studies', 'display_name': 'Generic Hybrid Ligand Binding Assay Liquid Chromatography High-Resolution Mass Spectrometry-Based Workflow for Multiplexed Human Immunoglobulin G1 Quantification at the Intact Protein Level: Application to Preclinical Pharmacokinetic Studies', 'publication_year': 2017, 'publication_date': '2017-01-24', 'ids': {'openalex': 'https://openalex.org/W2580906481', 'doi': 'https://doi.org/10.1021/acs.analchem.6b04997', 'mag': '2580906481', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/28192936'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1021/acs.analchem.6b04997', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S191963794', 'display_name': 'Analytical Chemistry', 'issn_l': '0003-2700', 'issn': ['0003-2700', '1520-6882'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320006', 'host_organization_name': 'American Chemical Society', 'host_organization_lineage': ['https://openalex.org/P4310320006'], 'host_organization_lineage_names': ['American Chemical Society'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5039483691', 'display_name': 'Christian Lanshoeft', 'orcid': 'https://orcid.org/0000-0002-2405-0227'}, 'institutions': [{'id': 'https://openalex.org/I1283582996', 'display_name': 'Novartis (Switzerland)', 'ror': 'https://ror.org/02f9zrr09', 'country_code': 'CH', 'type': 'company', 'lineage': ['https://openalex.org/I1283582996']}, {'id': 'https://openalex.org/I4400600974', 'display_name': 'Novartis Institutes for BioMedical Research', 'ror': 'https://ror.org/053gv2m95', 'country_code': None, 'type': None, 'lineage': ['https://openalex.org/I1283582996', 'https://openalex.org/I4400600974']}, {'id': 'https://openalex.org/I68947357', 'display_name': 'Université de Strasbourg', 'ror': 'https://ror.org/00pg6eq24', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I68947357']}, {'id': 'https://openalex.org/I1294671590', 'display_name': 'Centre National de la Recherche Scientifique', 'ror': 'https://ror.org/02feahw73', 'country_code': 'FR', 'type': 'government', 'lineage': ['https://openalex.org/I1294671590']}], 'countries': ['CH', 'FR'], 'is_corresponding': False, 'raw_author_name': 'Christian Lanshoeft', 'raw_affiliation_strings': ['Laboratoire\rde Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS, IPHC UMR 7178, 67000 Strasbourg, France', 'Novartis Institutes for Biomedical Research, Drug\rMetabolism and Pharmacokinetics, Novartis\rCampus, 4056 Basel, Switzerland'], 'affiliations': [{'raw_affiliation_string': 'Novartis Institutes for Biomedical Research, Drug\rMetabolism and Pharmacokinetics, Novartis\rCampus, 4056 Basel, Switzerland', 'institution_ids': ['https://openalex.org/I1283582996', 'https://openalex.org/I4400600974']}, {'raw_affiliation_string': 'Laboratoire\rde Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS, IPHC UMR 7178, 67000 Strasbourg, France', 'institution_ids': ['https://openalex.org/I68947357', 'https://openalex.org/I1294671590']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5066877921', 'display_name': 'Sarah Cianférani', 'orcid': 'https://orcid.org/0000-0003-4013-4129'}, 'institutions': [{'id': 'https://openalex.org/I68947357', 'display_name': 'Université de Strasbourg', 'ror': 'https://ror.org/00pg6eq24', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I68947357']}, {'id': 'https://openalex.org/I1294671590', 'display_name': 'Centre National de la Recherche Scientifique', 'ror': 'https://ror.org/02feahw73', 'country_code': 'FR', 'type': 'government', 'lineage': ['https://openalex.org/I1294671590']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Sarah Cianférani', 'raw_affiliation_strings': ['Laboratoire\rde Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS, IPHC UMR 7178, 67000 Strasbourg, France'], 'affiliations': [{'raw_affiliation_string': 'Laboratoire\rde Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS, IPHC UMR 7178, 67000 Strasbourg, France', 'institution_ids': ['https://openalex.org/I68947357', 'https://openalex.org/I1294671590']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5086307440', 'display_name': 'Olivier Heudi', 'orcid': 'https://orcid.org/0000-0003-3125-4639'}, 'institutions': [{'id': 'https://openalex.org/I1283582996', 'display_name': 'Novartis (Switzerland)', 'ror': 'https://ror.org/02f9zrr09', 'country_code': 'CH', 'type': 'company', 'lineage': ['https://openalex.org/I1283582996']}, {'id': 'https://openalex.org/I4400600974', 'display_name': 'Novartis Institutes for BioMedical Research', 'ror': 'https://ror.org/053gv2m95', 'country_code': None, 'type': None, 'lineage': ['https://openalex.org/I1283582996', 'https://openalex.org/I4400600974']}], 'countries': ['CH'], 'is_corresponding': False, 'raw_author_name': 'Olivier Heudi', 'raw_affiliation_strings': ['Novartis Institutes for Biomedical Research, Drug\rMetabolism and Pharmacokinetics, Novartis\rCampus, 4056 Basel, Switzerland'], 'affiliations': [{'raw_affiliation_string': 'Novartis Institutes for Biomedical Research, Drug\rMetabolism and Pharmacokinetics, Novartis\rCampus, 4056 Basel, Switzerland', 'institution_ids': ['https://openalex.org/I1283582996', 'https://openalex.org/I4400600974']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 4, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 2.749, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 44, 'citation_normalized_percentile': {'value': 0.999983, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 95, 'max': 96}, 'biblio': {'volume': '89', 'issue': '4', 'first_page': '2628', 'last_page': '2635'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11124', 'display_name': 'Protein purification and stability', 'score': 0.9997, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11124', 'display_name': 'Protein purification and stability', 'score': 0.9997, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11016', 'display_name': 'Monoclonal and Polyclonal Antibodies Research', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/2741', 'display_name': 'Radiology, Nuclear Medicine and Imaging'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T10683', 'display_name': 'Mass Spectrometry Techniques and Applications', 'score': 0.9987, 'subfield': {'id': 'https://openalex.org/subfields/1607', 'display_name': 'Spectroscopy'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/streptavidin', 'display_name': 'Streptavidin', 'score': 0.45625386}], 'concepts': [{'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.8967978}, {'id': 'https://openalex.org/C43617362', 'wikidata': 'https://www.wikidata.org/wiki/Q170050', 'display_name': 'Chromatography', 'level': 1, 'score': 0.7423475}, {'id': 'https://openalex.org/C162356407', 'wikidata': 'https://www.wikidata.org/wiki/Q180809', 'display_name': 'Mass spectrometry', 'level': 2, 'score': 0.6380326}, {'id': 'https://openalex.org/C204628709', 'wikidata': 'https://www.wikidata.org/wiki/Q864854', 'display_name': 'Biotinylation', 'level': 2, 'score': 0.6315537}, {'id': 'https://openalex.org/C9893847', 'wikidata': 'https://www.wikidata.org/wiki/Q1425625', 'display_name': 'Reproducibility', 'level': 2, 'score': 0.5359609}, {'id': 'https://openalex.org/C2776190903', 'wikidata': 'https://www.wikidata.org/wiki/Q421817', 'display_name': 'Streptavidin', 'level': 3, 'score': 0.45625386}, {'id': 'https://openalex.org/C207001950', 'wikidata': 'https://www.wikidata.org/wiki/Q141124', 'display_name': 'In vivo', 'level': 2, 'score': 0.42331252}, {'id': 'https://openalex.org/C64489805', 'wikidata': 'https://www.wikidata.org/wiki/Q873864', 'display_name': 'Liquid chromatography–mass spectrometry', 'level': 3, 'score': 0.4181636}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.115918994}, {'id': 'https://openalex.org/C2778204606', 'wikidata': 'https://www.wikidata.org/wiki/Q181354', 'display_name': 'Biotin', 'level': 2, 'score': 0.097127676}, {'id': 'https://openalex.org/C150903083', 'wikidata': 'https://www.wikidata.org/wiki/Q7108', 'display_name': 'Biotechnology', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D007074', 'descriptor_name': 'Immunoglobulin G', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': True}, {'descriptor_ui': 'D008024', 'descriptor_name': 'Ligands', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D053719', 'descriptor_name': 'Tandem Mass Spectrometry', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D019921', 'descriptor_name': 'Biotinylation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002851', 'descriptor_name': 'Chromatography, High Pressure Liquid', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004353', 'descriptor_name': 'Drug Evaluation, Preclinical', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005260', 'descriptor_name': 'Female', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006207', 'descriptor_name': 'Half-Life', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007118', 'descriptor_name': 'Immunoassay', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007074', 'descriptor_name': 'Immunoglobulin G', 'qualifier_ui': 'Q000097', 'qualifier_name': 'blood', 'is_major_topic': False}, {'descriptor_ui': 'D007074', 'descriptor_name': 'Immunoglobulin G', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D007074', 'descriptor_name': 'Immunoglobulin G', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010455', 'descriptor_name': 'Peptides', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D010455', 'descriptor_name': 'Peptides', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D051381', 'descriptor_name': 'Rats', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017208', 'descriptor_name': 'Rats, Wistar', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015203', 'descriptor_name': 'Reproducibility of Results', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1021/acs.analchem.6b04997', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S191963794', 'display_name': 'Analytical Chemistry', 'issn_l': '0003-2700', 'issn': ['0003-2700', '1520-6882'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320006', 'host_organization_name': 'American Chemical Society', 'host_organization_lineage': ['https://openalex.org/P4310320006'], 'host_organization_lineage_names': ['American Chemical Society'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/28192936', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [{'funder': 'https://openalex.org/F4320332736', 'funder_display_name': 'Novartis Pharma', 'award_id': None}], 'datasets': [], 'versions': [], 'referenced_works_count': 31, 'referenced_works': ['https://openalex.org/W1799710304', 'https://openalex.org/W1928475686', 'https://openalex.org/W1964595219', 'https://openalex.org/W1981862650', 'https://openalex.org/W1985092206', 'https://openalex.org/W1995855324', 'https://openalex.org/W2002778277', 'https://openalex.org/W2014590342', 'https://openalex.org/W2023161661', 'https://openalex.org/W2025186347', 'https://openalex.org/W2025704806', 'https://openalex.org/W2039037168', 'https://openalex.org/W2057067087', 'https://openalex.org/W2067408452', 'https://openalex.org/W2147568254', 'https://openalex.org/W2167098157', 'https://openalex.org/W2179158129', 'https://openalex.org/W2188074035', 'https://openalex.org/W2236039701', 'https://openalex.org/W2275444767', 'https://openalex.org/W2285473873', 'https://openalex.org/W2318841483', 'https://openalex.org/W2336611928', 'https://openalex.org/W2415133986', 'https://openalex.org/W2482733332', 'https://openalex.org/W2512408530', 'https://openalex.org/W2516877829', 'https://openalex.org/W2547189905', 'https://openalex.org/W2554469485', 'https://openalex.org/W2566223039', 'https://openalex.org/W2571186180'], 'related_works': ['https://openalex.org/W3039043954', 'https://openalex.org/W2946160530', 'https://openalex.org/W2234941614', 'https://openalex.org/W2089877124', 'https://openalex.org/W2077708318', 'https://openalex.org/W2025476836', 'https://openalex.org/W2011908365', 'https://openalex.org/W2010031434', 'https://openalex.org/W1989641713', 'https://openalex.org/W1968665113'], 'abstract_inverted_index': {'The': [0, 99, 114], 'quantitative': [1, 101, 199], 'analysis': [2, 139], 'of': [3, 120, 154, 165, 188], 'human': [4], 'immunoglobulin': [5], 'G1': [6], '(hIgG1)': [7], 'by': [8, 33, 92, 192], 'mass': [9, 42], 'spectrometry': [10, 43], 'is': [11, 31], 'commonly': [12], 'performed': [13], 'using': [14], 'surrogate': [15, 137], 'peptides': [16], 'after': [17], 'enzymatic': [18], 'digestion.': [19], 'Since': [20], 'some': [21], 'limitations': [22], 'are': [23], 'associated': [24], 'with': [25, 39, 69, 151], 'this': [26], 'approach,': [27], 'a': [28, 57, 70, 80, 155, 197], 'novel': [29], 'workflow': [30, 102, 143], 'presented': [32], 'hybridizing': [34], 'ligand': [35], 'binding': [36], 'assay': [37, 115], '(LBA)': [38], 'liquid': [40], 'chromatography-high-resolution': [41], '(LC-HRMS)': [44], 'for': [45, 162, 184, 202], 'hIgG1': [46, 190], 'quantification': [47, 164, 191], 'directly': [48], 'at': [49], 'the': [50, 87, 109, 132, 152, 169, 180, 185], 'intact': [51, 189], 'protein': [52], 'level.': [53], 'Different': [54], 'hIgG1s,': [55], 'including': [56], '[13C]-labeled': [58], 'version': [59], 'used': [60], 'as': [61, 196], 'internal': [62], 'standard,': [63], 'were': [64, 144, 173], 'immuno-enriched': [65], 'from': [66, 108], 'rat': [67], 'serum': [68], 'fully': [71], 'automated': [72], 'platform': [73], 'based': [74, 135], 'on': [75, 136], 'streptavidin': [76], 'coated': [77], 'tips': [78], 'and': [79, 127, 140, 148], 'biotinylated': [81], 'mouse': [82], 'anti-hIgG': [83], 'capture': [84], 'antibody': [85], 'targeting': [86], 'fragment': [88], 'crystallizable': [89], 'region': [90], 'followed': [91], 'overnight': [93], 'deglycosylation': [94], 'prior': [95], 'to': [96], 'LC-HRMS': [97], 'analysis.': [98], 'proposed': [100, 142], 'utilized': [103], 'extracted': [104], 'ion': [105], 'chromatograms': [106], '(XICs)': [107], 'nondeconvoluted': [110], 'full-scan': [111], 'MS': [112], 'spectrum.': [113], 'was': [116], 'validated': [117], 'in': [118, 146, 149], 'terms': [119], 'selectivity,': [121], 'sensitivity,': [122], 'accuracy/precision,': [123], 'carry-over,': [124], 'dilution': [125], 'linearity,': [126], 'reproducibility.': [128], 'Consistent': [129], 'data': [130], 'between': [131], 'conventional': [133], 'approach': [134], 'peptide': [138], 'our': [141, 177], 'obtained': [145], 'vitro': [147], 'vivo': [150], 'advantage': [153], 'less': [156], 'extensive': [157], 'sample': [158, 172], 'pretreatment.': [159], 'Multiplexing': [160], 'capabilities': [161], 'simultaneous': [163], 'different': [166], 'hIgG1s': [167], 'within': [168], 'same': [170], 'spiked': [171], 'also': [174, 195], 'exemplified.': [175], 'Altogether': [176], 'results': [178], 'pave': [179], 'way': [181], 'not': [182], 'only': [183], 'thorough': [186], 'application': [187], 'LBA-LC-HRMS': [193], 'but': [194], 'generic': [198], 'analytical': [200], 'method': [201], 'other': [203], 'hIgG': [204], 'isotypes': [205], 'or': [206], 'next': [207], 'generation': [208], 'biotherapeutics.': [209]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2580906481', 'counts_by_year': [{'year': 2024, 'cited_by_count': 3}, {'year': 2023, 'cited_by_count': 3}, {'year': 2022, 'cited_by_count': 6}, {'year': 2021, 'cited_by_count': 5}, {'year': 2020, 'cited_by_count': 9}, {'year': 2019, 'cited_by_count': 7}, {'year': 2018, 'cited_by_count': 10}, {'year': 2017, 'cited_by_count': 1}], 'updated_date': '2024-12-15T03:12:46.124153', 'created_date': '2017-02-03'}