Title: Utilizing of electrochemical detection coupled with liquidchromatography for isoflavones detection
Abstract:Among the biologically important roles of isoflavones is also
their effect on carcinogenesis. We used flow injection analysis
and high performance liquid W with electrochemical detection to
simultaneo...Among the biologically important roles of isoflavones is also
their effect on carcinogenesis. We used flow injection analysis
and high performance liquid W with electrochemical detection to
simultaneously determine certain isoflavones (biochanin A,
formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein
and genistein). The most suitable chromatographic conditions
were: mobile phase: 0.2 mol L--1 acetate buffer (pH 5.0); flow
rate 2.0 mL min--1; column and detector temperature: 26 C;
detection potential: 800 mV. Under the optimal conditions, the
detection limits were in the range of several ng mL--1. Their
simultaneous determination takes 15 min.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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