Title: Evaluation of Anticonvulsant Activities of Eryngium caucasicum with Maximal Electroshock and Kindling Model of Seizure in Mice
Abstract:Background: Eryngium species is reported to have variety of activities and has been used in traditional medicine for many years. In spite of the CNS and antioxidant activity of E. caucasicum, nothing ...Background: Eryngium species is reported to have variety of activities and has been used in traditional medicine for many years. In spite of the CNS and antioxidant activity of E. caucasicum, nothing is known about its anticonvulsant activity. Objectives: In this study, we aimed to evaluate the anticonvulsant activities of methanolic extracts of E. caucasicum on maximal electroshock (MES) and pentylenetetrazole (PTZ)-induced seizures in mice. Materials and Methods: The anticonvulsant activities of methanolic and polyphenolic extracts of E. caucasicum inflorescence at doses of 250, 500 and 1000 mg kg-1 were determined by Pentylenetetrazole (PTZ) and maximal electroshock (MES)-induced convulsions in mice. Results: Normal saline treated mice showed tonic hind limb extension for duration of 12.35 ± 0.46 seconds in the MES model. Methanolic extract at 250 and 500 mg kg-1 and polyphenolic extract at 200 mg kg-1 induced maximum protection against seizures, and polyphenolic extract at 200 mg kg-1 had 66.6% protection. PTZ (100 mg kg-1) induced tonic seizures in all the control mice. The most rapid onset of seizure relating to polyphenolic extract (50 mg kg-1) before PTZ was 9.59 ± 23.56 seconds and it was 103.0 ± 6.36 seconds at 200 mg kg-1. Conclusions: E. caucasicum methanolic and polyphenolic extracts had an antiepileptic effect against seizures induced by PTZ in mice, but the effect of polyphenolic extract was more potent.Read More