Title: Anthurium andraeanum (Linden ex André) Culture: In Vitro and Ex Vitro
Abstract:In order to optimize the establishment of both in vitro and ex vitro cultures of Anthurium andraeanum, recently expanded leaves from 3to 4-year-old greenhouse plants were disinfected with 1.43% NaOCl ...In order to optimize the establishment of both in vitro and ex vitro cultures of Anthurium andraeanum, recently expanded leaves from 3to 4-year-old greenhouse plants were disinfected with 1.43% NaOCl and treated in an antioxidant solution with 150 mg.L -1 citric acid, 100 mg.L -1 ascorbic acid and 200 mg.L -1 cysteine. Callus formation was induced from disinfected leaf explants ( 1 cm 2 ) on half Murashige and Skoog (MS/2) medium supplemented with 0.08 mg.L -1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg.L -1 6-benzylaminopurine (BAP), with and without 1 mg.L -1 N6-isopentenyl adenine (2iP), while shoots were induced to root on MS medium with BAP (0.0; 0.5 or 1.0 mg.L -1 ). Somaclonal variation was not observed. Organic soil, or vermiculite, or both (1:1), or both combined with sand, xaxim, sphagnum, pine cone, carbonized rice hull, turf, or sawdust (1:1:1), were used to test the efficacy of ex vitro acclimatization. This protocol resulted in the production of regenerants in about 120 days from the in vitro incubation of foliar explants to the ex vitro establishment of plantlets: callus was formed after 56 days of incubation; aerial buds and roots appeared after 28 days on regeneration medium, and the first leaves, after 33 days; after 70 days, 3 to 4 cordiform and dark green leaves were observed. MS/2 medium + 0.08 mg.L -1 2,4-D + 1 mg.L -1 BAP + 1 mg.L -1 2iP for callus induction, and MS + BAP 0.5 mg.L -1 for regenerating shoots are important protocol steps. Successful ex vitro growth was achieved within 7 months on all substrates with the exception of that containing sawdust.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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Cited By Count: 21
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