Title: Alkali pretreatment and enzymatic saccharification of oil palm empty fruit bunch fiber for production of ethanol
Abstract: Alkali pretreatment of oil palm empty fruit bunch (EFB) fiber was conducted to improve enzymatic saccharification of EFB fiber for ethanol production. EFB as one of the major biomass wastes from palm oil industry is a complex lignocellulosic material consists of 41.3 – 46.5% cellulose, 25.3 – 33.8% hemicellulose and 27.6 – 32.5% lignin. Alkali pretreatment of EFB using NaOH 1 N with varied temperatures 30 and 60 0 C and reaction times 30, 60, 90, 120 and 150 minutes were investigated. Furthermore, enzymatic saccharification of pretreated EFB was examined. The pretreated substrate was subjected to an enzymatic saccharification using meicelase (10, 20 and 40 FPU/g substrate) at 40 0 C, pH 4.5, 100 rpm for conversion of cellulose and hemicellulose contained in palm oil EFB to monomeric sugars. The alkali pretreatment of EFB using NaOH can significantly improve enzymatic saccharification of EFB by removing more lignin and hemicellulose and increasing its accessibility to hydrolytic enzymes. The results showed optimum pretreatment condition was NaOH 1 N at 30 0 C and 90 minutes with optimum component loss of lignin and hemicellulose was respectively 45.8 % and 35.6 %. Saccharification of EFB pretreated by NaOH 1 N (at 30 0 C and 90 minutes) for 45 hours and pH 4.5 result in optimum glucose and xylose concentration 19.95 mg/ml and 25.24 mg/ml respectively.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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