Title: Diffusion measured with scanning fluorescence correlation spectroscopy
Abstract: Diffusion coefficient is a physical parameter that can be used to draw conclusions about the size and shape of the diffusing species and about the properties of the environment in which diffusion occurs. One of the methods to measure the diffusion coefficient of fluorescent or fluorescently labelled molecules is Fluorescence Correlation Spectroscopy (FCS). The diffusion coefficient is derived from the measured diffusion time the characteristic time a molecule needs to diffuse through an observation volume of a known size. The diffusion time is obtained from an autocorrelation of the measured fluctuating fluorescence signal by fitting it to a model which assumes a well-defined observation volume. The small observation volume is experimentally realized by tight focusing of the excitation light combined with confocal detection of fluorescence, as known from confocal laser scanning microscopy.
Publication Year: 2009
Publication Date: 2009-12-31
Language: en
Type: article
Indexed In: ['crossref']
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