Publication Information

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'related_works': [], 'abstract_inverted_index': {'目的：利用': [0], 'RNAi': [1], '（RNA': [2], 'interference，': [3], 'RNAi）技术抑制食管癌': [4], 'Eca': [5, 11, 19, 23, 26, 28, 37, 43, 46, 59, 70], '－109细胞': [6], 'survivin': [7, 9, 13, 21, 33, 41, 64, 68], '基因表达，观察': [8], '基因沉默后对': [10], '－109细胞化疗敏感性的影响。方法构建靶向': [12], '基因特定序列的小干扰': [14], 'RNA（small': [15], 'interference': [16], 'RNA，': [17], 'siRNA）真核表达载体并转染': [18], '－109细胞，筛选得到稳定转染的': [20], '干扰组细胞': [22, 52], '－109／si': [24, 38, 44, 50, 60], '－survivin，同时设转染无关干扰质粒的': [25], '－109细胞为阴性对照组，未转染的': [27], '－109细胞为空白对照组；通过': [29], 'Western': [30], 'Blot': [31], '法检测干扰前后': [32], '基因沉默抑制效果；随后将各组细胞与不同浓度的氟尿嘧啶（5－Fu）作用，MTT': [34], '法检测各组细胞对5－Fu': [35], '的半数抑制浓度（IC50），流式细胞仪分析各组细胞的凋亡率。结果干扰组': [36], '－survivin': [39, 51, 61], '细胞': [40], '蛋白表达水平（18．75±3．12）较阴性对照组': [42], '－control（44．17±3．15）及空白对照组': [45], '－109（46．20±2．62）明显下调（P': [47], '＜0．05）；联合应用5－Fu': [48, 57], '的Eca': [49], 'IC50为（18．75±1．53）mg·': [53], 'L－1，显著低于空白对照组（39．65±1．75）mg·': [54], 'L－1和阴性对照组（38．95±1．34）mg·': [55], 'L－1，差异具有统计学意义（P': [56], '的': [58, 65], '干扰组细胞凋亡率为（37．35±1．52）％，明显高于空白对照组（11．26±1．21）％和阴性对照组（12．34±1．32）％，差异具有显著性（P': [62], '＜0．05）。结论靶向': [63], 'siRNA': [66], '能特异性沉默': [67], '基因表达，抑制细胞增殖，诱导细胞凋亡，并增强人食管癌': [69], '－109细胞对5－Fu': [71], '的化疗敏感性。': [72]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2521562244', 'counts_by_year': [], 'updated_date': '2024-12-14T19:19:56.779566', 'created_date': '2016-09-30'}