Title: The Enzymatic Degradation of Heme Proteins into Bile Pigments. X (2)
Abstract: 1. Several properties of the activator of liver heme α-methenyl oxygenase are described.2. Catalase did not inhibit the activated enzyme activity, suggesting that the activator does not merely regenerate the peroxide or a peroxide forming system.3. The heme α-methenyl oxygenase activity as a function of activator and enzyme concentration was observed. It is evident that the activator can not afford to be effective on dialyzed enzyme.4. The short lag period of 656mμ substance formation, which was observed in the partially purified heme α-methenyl oxygenase assay, was completely eliminated by the addition of activator in the routine assay mixture.