Abstract: Phase contrast provides a form of transmitted light microscopy whereby images of high contrast and resolution can be obtained from transparent biological samples. The contrast formation is based on exploiting the phase shift of waves passing through a sample even though little or no absorption-based contrast is provided. These small phase shifts are then converted by the optical elements of the imaging system into larger relative shifts to provide higher interference, intensity change, and, therefore, contrast. This chapter outlines the principles of phase contrast as it is used today in standard commercial microscopes and outlines some biological uses.
Publication Year: 2012
Publication Date: 2012-10-26
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 5
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