Abstract:This chapter helps students to grow two legume crops with no inoculum, correct inoculum, and incorrect inoculum to study symbiotic nitrogen fixation by Rhizobium bacteria. The plant growth medium (ver...This chapter helps students to grow two legume crops with no inoculum, correct inoculum, and incorrect inoculum to study symbiotic nitrogen fixation by Rhizobium bacteria. The plant growth medium (vermiculite, perlite, or washed sand) is inert, and all essential elements except nitrogen are supplied in a nutrient solution. Students should be able to observe nitrogen deficiency symptoms, nodule formation and activity, and the effect of using the correct and incorrect strains of Rhizobium. The chapter demonstrates the effect of inoculation on nodule formation and resultant growth of legume plants. Soybean, alfalfa, garden bean, and pea are all in separate cross-inoculation groups and require different strains of bacteria. Nitrogen in the nutrient solution is withheld to demonstrate that legumes cannot produce nitrogen without nodules, and to show that legumes can obtain plenty of nitrogen from nodules if they are present and active.Read More
Publication Year: 2013
Publication Date: 2013-08-12
Language: en
Type: book-chapter
Indexed In: ['crossref']
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