Title: Electrokinetic Lift Effects on the Separation Factor in Capillary Hydrodynamic Fractionation
Abstract: Above an eluant ionic strength of 10-4 M (typical of normal CHDF operation), the normalized average residence time of monodisperse polystyrene nanospheres (average diameter < 0.36 μm) being pumped under laminar flow conditions is independent of the flow rate. At lower ionic strength, however, a significant flow rate dependence has been observed using the same latexes. This behavior is inconsistent with an inertial lift force mechanism and supports the hypothesis that the anomalous particle elution was electrokinetically induced. The incorporation of a total electrokinetic lift force expression into our mathematical model has improved the prediction of the separation factor (a relative particle average velocity), indicating that the shear flow contribution to the lift force is significant in the CHDF simulation. Particle separation experiments have demonstrated that the use of very low conductivity eluants in CHDF provides better resolution of colloidal particles than that presently obtained using higher electrolyte concentrations.
Publication Year: 1998
Publication Date: 1998-06-10
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 2
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