Title: Selective Screening Methods for the Isolation of High Yielding Cellulase Mutants of <i>Trichoderma reesei</i>
Abstract: Screening methods for the selection of high-yielding cellulase mutants of Trichoderma reesei have been devised that specifically detect hyperproducing, catabolite repression-resistant, and end-product inhibition-resistant strains. Individual selection techniques employing specific substrates and end-product inhibitors allow for visual screening of a single enzyme in the cellulase complex: endo-β 1-4 glucanase and β-glucosidase (cellobiase) as well as the synergistic action of all the enzymes of the cellulase complex. By use of these methods, mutant strains have been isolated that hyperproduce cellulase and are resistant to catabolite repression by glucose or glycerol.
Publication Year: 1979
Publication Date: 1979-06-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 247
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