Title: NMR Structural Biology Using Paramagnetic Lanthanide Probe
Abstract:We describe the recent development in nuclear magnetic resonance (NMR) equipped with paramagnetic lanthanide probe. Paramagnetic lanthanide probe provides long-range (~40 Å) distance and angular infor...We describe the recent development in nuclear magnetic resonance (NMR) equipped with paramagnetic lanthanide probe. Paramagnetic lanthanide probe provides long-range (~40 Å) distance and angular information that can be exploited in structure determination of large proteins and their complexes, dynamics, ligand screening, and structure-based resonance assignment. Application of the paramagnetic lanthanide probe is not limited to metal-binding proteins but becoming general by the use of lanthanide-binding tags. We here illustrate the practical aspects of the experiments and analyses for the use of paramagnetic lanthanide probe. Applications to protein-protein and protein-ligand structure determination and ligand screening are also shown.Read More
Publication Year: 2016
Publication Date: 2016-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 3
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