Title: EFFECTS ON <i>STRIGA</i> PARASITISM OF TRANSGENIC MAIZE ARMED WITH RNAi CONSTRUCTS TARGETING ESSENTIAL <i>S. ASIATICA</i> GENES
Abstract: Integrating New Technologies for Striga Control, pp. 185-196 (2007) No AccessEFFECTS ON STRIGA PARASITISM OF TRANSGENIC MAIZE ARMED WITH RNAi CONSTRUCTS TARGETING ESSENTIAL S. ASIATICA GENESAnic de Framond, Patrick J. Rich, John McMillan, and Gebisa EjetaAnic de FramondSyngenta Biotechnology Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709-2257, USA, Patrick J. RichPurdue University, Department of Agronomy, 915 W. State St., West Lafayette, IN 47907-2054, USA, John McMillanSyngenta Biotechnology Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709-2257, USA, and Gebisa EjetaPurdue University, Department of Agronomy, 915 W. State St., West Lafayette, IN 47907-2054, USAhttps://doi.org/10.1142/9789812771506_0014Cited by:24 PreviousNext AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsRecommend to Library ShareShare onFacebookTwitterLinked InRedditEmail Abstract: We are attempting to engineer transgenic maize for resistance to the parasitic weed Striga, based on RNA interference silencing technology (RNAi). In this approach, the transgenic maize produces double-stranded RNA (dsRNA) molecules targeted against genes essential for Striga survival. As Striga establishes on the maize roots, the silencing agent could systemically spread to its cells, shutting down the targeted essential genes and thereby killing the parasite. Five Striga genes were chosen as targets in either of 13 dsRNA interference constructs and 55 transgenic maize lines containing an RNAi construct were selected for testing. We report the results of an initial screening of these materials with S. asiatica. Although some events still need to be assayed, none of the transgenic maize in 11 events tested is obviously resistant to Striga parasitism within 4-5 weeks of infestation. Some Striga plants were able to develop and survive on all transgenic materials tested. There are indications that Striga grows slower when attached to maize with an RNAi construct in at least half the transformation events tested relative to non-transgenic segregants of those events. Further testing is needed to confirm these results, and ascertain their field relevance. 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Publication Year: 2007
Publication Date: 2007-06-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
Access and Citation
Cited By Count: 31
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