Title: Expression of mitochondrial DNA in : the construction of sets of isonuclear haploid strains containing different specified mitochondrial genomes
Abstract: In order to perform systematic biochemical studies of the expression of the mitochondrial genome in different strains of Saccharomycescerevisiae, we have constructed a series of haploid strains in which a specified mitochondrial genome has been placed in cells containing a particular nuclear genome. The desired strains were obtained from crosses between rho+ and rhoo haploids, one of which carried the karl-l mutation which interferes with nuclear fusion. These newly constructed strains provide the basis for studying mitochondrial gene expression as a function of (i) the nucleotide sequence differences that are apparent in the mitochondrial DNAs from different strains, and (ii) the role played by different nuclear genomes in modulating the expression of mitochondrial genes.
Publication Year: 1978
Publication Date: 1978-11-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 29
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