Title: Effect of fish meal replacement by <i>Chlorella</i> meal with dietary cellulase addition on growth performance, digestive enzymatic activities, histology and myogenic genes’ expression for crucian carp <i>Carassius auratus</i>
Abstract: Aquaculture ResearchVolume 48, Issue 6 p. 3244-3256 Original Article Effect of fish meal replacement by Chlorella meal with dietary cellulase addition on growth performance, digestive enzymatic activities, histology and myogenic genes’ expression for crucian carp Carassius auratus Xi Shi, Xi Shi Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, ChinaSearch for more papers by this authorZhi Luo, Corresponding Author Zhi Luo [email protected][email protected] Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, China Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, ChinaCorrespondence: Prof. Z Luo, College of Fisheries, Huazhong Agricultural University, No. 1, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, China. E-mails: [email protected]; [email protected] for more papers by this authorFeng Chen, Feng Chen Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, ChinaSearch for more papers by this authorChuan-Chuan Wei, Chuan-Chuan Wei Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, ChinaSearch for more papers by this authorKun Wu, Kun Wu Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, ChinaSearch for more papers by this authorXiao-Ming Zhu, Xiao-Ming Zhu State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaSearch for more papers by this authorXu Liu, Xu Liu Panjin Guanghe Fishery Ltd., Co., Panjin, ChinaSearch for more papers by this author Xi Shi, Xi Shi Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, ChinaSearch for more papers by this authorZhi Luo, Corresponding Author Zhi Luo [email protected][email protected] Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, China Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, ChinaCorrespondence: Prof. Z Luo, College of Fisheries, Huazhong Agricultural University, No. 1, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, China. E-mails: [email protected]; [email protected] for more papers by this authorFeng Chen, Feng Chen Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, ChinaSearch for more papers by this authorChuan-Chuan Wei, Chuan-Chuan Wei Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, ChinaSearch for more papers by this authorKun Wu, Kun Wu Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, ChinaSearch for more papers by this authorXiao-Ming Zhu, Xiao-Ming Zhu State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaSearch for more papers by this authorXu Liu, Xu Liu Panjin Guanghe Fishery Ltd., Co., Panjin, ChinaSearch for more papers by this author First published: 13 July 2016 https://doi.org/10.1111/are.13154Citations: 46Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract This study was conducted to investigate the effects of fish meal (FM) replacement by Chlorella meal (CM) with dietary cellulase supplementation on growth performance, digestive enzymatic activities, histology and myogenic genes’ expression in crucian carp Carassius auratus (initial body weight: 2.90 ± 0.02 g, mean ± SEM). Six isonitrogenous diets were formulated at two cellulase levels (0 and 2 g kg−1). At each cellulase level, CM was added at three levels of 0, 533.1 and 710.8 g kg−1 to substitute 0, 75 and 100% of dietary FM respectively. Each experimental diet was randomly assigned to triplicate groups with 25 juvenile fish per fibreglass tank for 8 weeks. Dietary CM substitution significantly increased growth, feed utilization, amylase activity and the expression of Myod, Mrf4 and Myf5, but reduced the Myog expression. Dietary cellulase addition increased hepatosomatic and viscerosomatic index, lipase activity and the expression of Mrf4, but reduced trypsin activity and the expression of Myog and Myf5. Dietary CM substitution enlarged the cell size and also caused some karyopyknosis in liver. Our results showed that CM could totally replace FM in diets; dietary cellulase supplementation at the level of 2 g kg−1 played a subtle role in improving growth and feed utilization for crucian carp. Citing Literature Volume48, Issue6June 2017Pages 3244-3256 RelatedInformation