Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2469653072', 'doi': 'https://doi.org/10.2144/96211bm10', 'title': 'Preparation of Single-Stranded Phagemid DNA Without Chromosomal DNA Contamination', 'display_name': 'Preparation of Single-Stranded Phagemid DNA Without Chromosomal DNA Contamination', 'publication_year': 1996, 'publication_date': '1996-07-01', 'ids': {'openalex': 'https://openalex.org/W2469653072', 'doi': 'https://doi.org/10.2144/96211bm10', 'mag': '2469653072', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/8816234'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.2144/96211bm10', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S79990748', 'display_name': 'BioTechniques', 'issn_l': '0736-6205', 'issn': ['0736-6205', '1940-9818'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320316', 'host_organization_name': 'Future Science Ltd', 'host_organization_lineage': ['https://openalex.org/P4310320316'], 'host_organization_lineage_names': ['Future Science Ltd'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'doaj', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://doi.org/10.2144/96211bm10', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5080203607', 'display_name': 'Wei Tang', 'orcid': 'https://orcid.org/0000-0001-9558-072X'}, 'institutions': [{'id': 'https://openalex.org/I136199984', 'display_name': 'Harvard University', 'ror': 'https://ror.org/03vek6s52', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I136199984']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Wei Tang', 'raw_affiliation_strings': ['Harvard Medical School, Boston, MA, USA'], 'affiliations': [{'raw_affiliation_string': 'Harvard Medical School, Boston, MA, USA', 'institution_ids': ['https://openalex.org/I136199984']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5101616271', 'display_name': 'Jian‐Ning Liu', 'orcid': 'https://orcid.org/0000-0002-9796-8565'}, 'institutions': [{'id': 'https://openalex.org/I136199984', 'display_name': 'Harvard University', 'ror': 'https://ror.org/03vek6s52', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I136199984']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Jian-Ning Liu', 'raw_affiliation_strings': ['Harvard Medical School, Boston, MA, USA'], 'affiliations': [{'raw_affiliation_string': 'Harvard Medical School, Boston, MA, USA', 'institution_ids': ['https://openalex.org/I136199984']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5009650479', 'display_name': 'Victor Gurewich', 'orcid': 'https://orcid.org/0000-0002-2648-2349'}, 'institutions': [{'id': 'https://openalex.org/I136199984', 'display_name': 'Harvard University', 'ror': 'https://ror.org/03vek6s52', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I136199984']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Victor Gurewich', 'raw_affiliation_strings': ['Harvard Medical School Boston, MA, USA'], 'affiliations': [{'raw_affiliation_string': 'Harvard Medical School Boston, MA, USA', 'institution_ids': ['https://openalex.org/I136199984']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 1415, 'currency': 'USD', 'value_usd': 1415, 'provenance': 'doaj'}, 'apc_paid': {'value': 1415, 'currency': 'USD', 'value_usd': 1415, 'provenance': 'doaj'}, 'fwci': 0.0, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 1, 'citation_normalized_percentile': {'value': 0.241307, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 54, 'max': 62}, 'biblio': {'volume': '21', 'issue': '1', 'first_page': '53', 'last_page': '54'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10207', 'display_name': 'Advanced biosensing and bioanalysis techniques', 'score': 0.9988, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10207', 'display_name': 'Advanced biosensing and bioanalysis techniques', 'score': 0.9988, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10725', 'display_name': 'RNA Interference and Gene Delivery', 'score': 0.9986, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10878', 'display_name': 'CRISPR and Genetic Engineering', 'score': 0.9933, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/phagemid', 'display_name': 'Phagemid', 'score': 0.54937994}], 'concepts': [{'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.5858381}, {'id': 'https://openalex.org/C10079403', 'wikidata': 'https://www.wikidata.org/wiki/Q424475', 'display_name': 'Phagemid', 'level': 5, 'score': 0.54937994}, {'id': 'https://openalex.org/C2993838110', 'wikidata': 'https://www.wikidata.org/wiki/Q494230', 'display_name': 'Medical school', 'level': 2, 'score': 0.5452493}, {'id': 'https://openalex.org/C22744801', 'wikidata': 'https://www.wikidata.org/wiki/Q172778', 'display_name': 'Plasmid', 'level': 3, 'score': 0.52981555}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.41182104}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.34939075}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.31230897}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.2585967}, {'id': 'https://openalex.org/C71924100', 'wikidata': 'https://www.wikidata.org/wiki/Q11190', 'display_name': 'Medicine', 'level': 0, 'score': 0.1667842}, {'id': 'https://openalex.org/C2776441376', 'wikidata': 'https://www.wikidata.org/wiki/Q165028', 'display_name': 'Bacteriophage', 'level': 4, 'score': 0.06620437}, {'id': 'https://openalex.org/C547475151', 'wikidata': 'https://www.wikidata.org/wiki/Q25419', 'display_name': 'Escherichia coli', 'level': 3, 'score': 0.0}, {'id': 'https://openalex.org/C509550671', 'wikidata': 'https://www.wikidata.org/wiki/Q126945', 'display_name': 'Medical education', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D001435', 'descriptor_name': 'Bacteriophages', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D004277', 'descriptor_name': 'DNA, Single-Stranded', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D004279', 'descriptor_name': 'DNA, Viral', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D001435', 'descriptor_name': 'Bacteriophages', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011232', 'descriptor_name': 'Chemical Precipitation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004277', 'descriptor_name': 'DNA, Single-Stranded', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004279', 'descriptor_name': 'DNA, Viral', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009691', 'descriptor_name': 'Nucleic Acid Denaturation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009695', 'descriptor_name': 'Nucleic Acid Renaturation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 3, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.2144/96211bm10', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S79990748', 'display_name': 'BioTechniques', 'issn_l': '0736-6205', 'issn': ['0736-6205', '1940-9818'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320316', 'host_organization_name': 'Future Science Ltd', 'host_organization_lineage': ['https://openalex.org/P4310320316'], 'host_organization_lineage_names': ['Future Science Ltd'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://doaj.org/article/d6b9e5524be2407baf87c55478871291', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306401280', 'display_name': 'DOAJ (DOAJ: Directory of Open Access Journals)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': None, 'host_organization_name': None, 'host_organization_lineage': [], 'host_organization_lineage_names': [], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/8816234', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.2144/96211bm10', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S79990748', 'display_name': 'BioTechniques', 'issn_l': '0736-6205', 'issn': ['0736-6205', '1940-9818'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320316', 'host_organization_name': 'Future Science Ltd', 'host_organization_lineage': ['https://openalex.org/P4310320316'], 'host_organization_lineage_names': ['Future Science Ltd'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'score': 0.58, 'display_name': 'Good health and well-being', 'id': 'https://metadata.un.org/sdg/3'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 1, 'referenced_works': ['https://openalex.org/W1982315729'], 'related_works': ['https://openalex.org/W4391250611', 'https://openalex.org/W4388965752', 'https://openalex.org/W2908588478', 'https://openalex.org/W2890718724', 'https://openalex.org/W2751496096', 'https://openalex.org/W2750385014', 'https://openalex.org/W2429129060', 'https://openalex.org/W2409844362', 'https://openalex.org/W2114870167', 'https://openalex.org/W2065991117'], 'abstract_inverted_index': {'BioTechniquesVol.': [0], '21,': [1, 116], 'No.': [2, 117], '1': [3, 118], 'BenchmarksOpen': [4], 'AccessPreparation': [5], 'of': [6, 47, 75, 103], 'Single-Stranded': [7, 76], 'Phagemid': [8, 77], 'DNA': [9, 12, 78, 81], 'Without': [10, 79], 'Chromosomal': [11, 80], 'ContaminationWei': [13], 'Tang,': [14], 'Jian-Ning': [15, 26], 'Liu': [16], '&': [17, 33], 'Victor': [18, 34, 38], 'GurewichWei': [19], 'TangHarvard': [20], 'Medical': [21, 28, 53, 59], 'School,': [22, 29, 54, 60], 'Boston,': [23, 30, 55, 61], 'MA,': [24, 31, 62], 'USA,': [25], 'LiuHarvard': [27], 'USA': [32], 'Gurewich*Address': [35], 'correspondence': [36], 'to': [37, 68], 'Gurewich,': [39], 'Vascular': [40], 'Research': [41], 'Laboratory,': [42], 'Institute': [43], 'for': [44, 124], 'the': [45, 101, 125], 'Prevention': [46], 'Cardiovascular': [48], 'Disease,': [49], 'Deaconess': [50], 'Hospital,': [51], 'Harvard': [52], 'MA': [56], '02215,': [57], 'USA.Harvard': [58], 'USAPublished': [63], 'Online:2': [64], 'Aug': [65], '2018https://doi.org/10.2144/96211bm10AboutSectionsPDF/EPUB': [66], 'ToolsAdd': [67], 'favoritesDownload': [69], 'CitationsTrack': [70], 'Citations': [71], 'ShareShare': [72], 'onFacebookTwitterLinkedInRedditEmail': [73], '"Preparation': [74], 'Contamination."': [82], ',': [83], '21(1),': [84], 'pp.': [85], '53–54FiguresReferencesRelatedDetailsCited': [86], 'ByA': [87], 'quantitative': [88], 'PCR-based': [89], 'assay': [90], 'reveals': [91], 'that': [92], 'nucleotide': [93], 'excision': [94], 'repair': [95], 'plays': [96], 'a': [97], 'predominant': [98], 'role': [99], 'in': [100, 139], 'removal': [102], 'DNA-protein': [104], 'crosslinks': [105], 'from': [106], 'plasmids': [107], 'transfected': [108], 'into': [109], 'mammalian': [110], 'cellsDNA': [111], 'Repair,': [112], 'Vol.': [113, 115], '62': [114], 'Follow': [119], 'us': [120], 'on': [121], 'social': [122], 'media': [123], 'latest': [126], 'updates': [127], 'Metrics': [128], 'Downloaded': [129], '103': [130], 'times': [131], 'History': [132], 'Published': [133, 138], 'online': [134], '2': [135], 'August': [136], '2018': [137, 144], 'print': [140], 'July': [141], '1996': [142], 'Information©': [143], 'Author(s)PDF': [145], 'download': [146]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2469653072', 'counts_by_year': [{'year': 2018, 'cited_by_count': 1}], 'updated_date': '2024-12-11T15:30:36.504220', 'created_date': '2016-07-22'}