Title: [Novel site-specific endonuclease from Acinetobacter species M strain].
Abstract: Site-specific endonuclease R. AspMI was isolated and purified to apparent functional homogeneity from Acinetobacter species (strain M). The enzyme recognizes symmetrical DNA sequence 5'-AGG decreases CCT-3' and cleaves it at the site indicated by the arrow forming blind DNA ends. The endonuclease is an isoschizomer of the StuI endonuclease. Cleavage of the DNA site was inhibited by dcm-methylation. AspMI is approximately equal to 30 kD monomer.
Publication Year: 1996
Publication Date: 1996-08-01
Language: en
Type: article
Indexed In: ['pubmed']
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Cited By Count: 2
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