Title: Use of post-thaw semen quality parameters to predict fertility of water buffalo (<i>Bubalus bubalis</i>) bull during peak breeding season
Abstract: AndrologiaVolume 49, Issue 4 e12639 ORIGINAL ARTICLE Use of post-thaw semen quality parameters to predict fertility of water buffalo (Bubalus bubalis) bull during peak breeding season H. Ahmed, H. Ahmed Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan Reproductive Physiology Laboratory, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, PakistanSearch for more papers by this authorS. M. H. Andrabi, Corresponding Author S. M. H. Andrabi [email protected] Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan Correspondence Syed. M. H. Andrabi, Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan. Email: [email protected] for more papers by this authorM. Anwar, M. Anwar Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, PakistanSearch for more papers by this authorS. Jahan, S. Jahan Reproductive Physiology Laboratory, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, PakistanSearch for more papers by this author H. Ahmed, H. Ahmed Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan Reproductive Physiology Laboratory, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, PakistanSearch for more papers by this authorS. M. H. Andrabi, Corresponding Author S. M. H. Andrabi [email protected] Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan Correspondence Syed. M. H. Andrabi, Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan. Email: [email protected] for more papers by this authorM. Anwar, M. Anwar Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, PakistanSearch for more papers by this authorS. Jahan, S. Jahan Reproductive Physiology Laboratory, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, PakistanSearch for more papers by this author First published: 12 July 2016 https://doi.org/10.1111/and.12639Citations: 19Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Summary This study was designed to predict the fertility of water buffalo bull using post-thaw semen quality parameters during peak breeding season. Thirty ejaculates were collected from five bulls with artificial vagina and cryopreserved. At post-thaw, semen was analysed for motility parameters, velocity distribution, kinematics, DNA integrity/fragmentation, viability, mitochondrial transmembrane potential, morphology, plasma membrane and acrosome integrity. Data of 514 inseminations were collected for estimation of in vivo fertility. Pearson's correlation coefficients showed that progressive motility (PM), rapid velocity, average path velocity, straight line velocity, straightness, supravital plasma membrane integrity, viable spermatozoon with intact acrosome or with high mitochondrial activity were correlated with in vivo fertility (r = .81, p < .01; r = .85, p < .01; r = .64, p < .05; r = .73, p < .05; r = .57, p < .05; r = .88, p < .01; r = .84, p < .01 and r = .81, p < .01 respectively). Step forward multiple regression analysis showed that the best single predictor of fertility was PM. However, combinations of semen quality parameters to predict fertility were better as compared to single parameter. In conclusion, fertility of buffalo bull can be predicted through some of the post-thaw in vitro semen quality tests during peak breeding season. Citing Literature Volume49, Issue4May 2017e12639 RelatedInformation