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Title: $Differential Responses of Buckwheat Leaf Cells to Growth Substances Stimulating Cell Division
Abstract: Isolated buckwheat cotyledons form calli, roots or buds when cultured in an appropriate medium. A medium containing high 2,4-D (5 mg 1−1) and low KN (01 mg I−1), which induces callus formation, was found to stimulate cell division in the layer between palisade and spongy parenchyma tissue after 72 h. Low 2,4-D and low KN (01 mg I−1 each), which stimulates root formation in buckwheat cotyledons, induces divisions primarily in spongy parenchyma cells. In a high benzylaminopurine (10−5 M) and a low IAA (10−6 M) medium, which favours bud induction, cell divisions were localized to the palisade layer. The differential responsiveness of leaf cells to various hormone treatments is discussed.