Title: EFFECT OF 50% ETHANOLIC EXTRACT OF PEGAGAN HERB (Centella asiatica (L.) Urban) ON CELL PROLIFERATION OF LYMPHOCYTES IN Balb/c MALE MICE INDUCED BY HEPATITIS B VACCINE PENGARUH EKSTRAK ETANOLIK 50% HERBA PEGAGAN (Centella asiatica (L.) Urban) TERHADAP PENINGKATAN PROLIFERASI SEL LIMFOSIT MENCIT JANTAN GALUR Balb/c YANG DIINDUKSI VAKSIN HEPATITIS B
Abstract:Pegagan (Centella asiatica (L.) Urban) has been known as multi effect plant that empirically used in the traditional medicine. This research aims to eva luate the effect of 50%ethanolic extract of peg...Pegagan (Centella asiatica (L.) Urban) has been known as multi effect plant that empirically used in the traditional medicine. This research aims to eva luate the effect of 50%ethanolic extract of pegagan herb against increased lymphocyte pro liferation cells in Balb/c mice that in duced with hepati tis B vaccine, and also to evaluate triterpen g licosida compound (asiaticosida) of 50% ethanolic extrac t of pegagan herb. The test was done by 60 Balb /c male mices that divided into 6 g roups : control group, testing group that was treated wi th pegagan extract dose of 25mg/KgBB, 50 mg/KgBB, 100mg/KgBB, 200mg/KgBB and 400mg/KgBB. All of mices were vaccinated on day 0 and days 15. Furthermore, identification of triterpen glicosida compound was done by KLT method using BAW 4:1:5 as upper eluen. Lymphocyte cells were isolated and the cells pro liferation measured by MTT reduction method. Data was analyzed statistically using 95% confidence level. The result sho wn that 50% of ethanolic extract of pegagan herb did not give the immuno modulatory effect while the identification test shown that 50% of extrac t of pegagan herb has triterpen g licosida compound (asiaticoside).Read More
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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