Title: Isolation and characterization of the gene encoding the rat α1B adrenergic receptor
Abstract: Using a rat α1B adrenergic receptor(AR)-encoding cDNA probe, we isolated two genomic clones from a rat liver genomic DNA library. Southern blot analysis and nucleotide sequencing indicate that the rat α1BAR gene has two exons and a single large intron of at least 16 kb. Analysis of the sequence of the 5'-flanking region suggests that this gene has the features of a housekeeping gene: it has neither a TATA box nor a CAAT box, but has multiple transcription start points (tsp), multiple Sp1-binding sites, and a high G + C content. The 5'-flanking region also contains consensus sequences for AP1-and AP2-binding sites, and putative sites for cyclic AMP (CRE), glucocorticoid (GRE), and thyroid responses (TRE). The 3'-flanking region contains a putative polyadenylation signal (ATTAAA) 492 bp downstream from the stop codon. A comparison of the rat α1BAR sequence with that of the recently cloned human α1BAR gene showed that the overall structure of this gene is highly conserved, with some notable differences in the 5'-flanking region.
Publication Year: 1993
Publication Date: 1993-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 56
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