Title: In Vitro Propagation through Seed Culture and Regeneration of Asparagus Densiflorus L. through Callus Cultures Derived from Hypocotyls
Abstract: A successful and reproducible clonal propagation pr otocol from seed culture and a high regeneration ability through cal lus cultures derived from hypocotyls of Asparagus densiflorus were achieved i n this investigation. The results showed that good seed germination could be achieved by cold and wet seed stratification prior to culture and using sloped su rface medium enriched with 2 mgl -1 BA. The use of hypocotyls was superior upon the use of radicals as explants for shoot multiplication. The best parameters for hypocotyl emultiplication were recorded while using BA alone at 1.0 mgl -1 by producing 8.13 shoots/ explant of 7.81 cm mean length and 33.00 leaves per explant. The be st auxin for rooting of Asparagus shoots was IBA at 1.25 mgl -1 as compared to IAA or NAA. At callus induction and initiation stage, a compact green callus was produc ed by the addition of 0.5+ 0.3 mgl -1 of both BA and NAA. The addition of 0.5+ 0.2 mgl -1 from BA and NAA gave the best callus regeneration ability. Using of BA and NAA at 0.5+ 0.2 gave the best shoots multiplication parameters regenerated from the call us. The highest number of roots (12.4 roots/ explant) and the longest roots (4.98 c m) were gained from the addition of 1.25 mgl -1 IBA. A high survival rate (95%) was found with Asp aragus plantlets gradually acclimatized into the out-air conditions.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 8
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