Abstract: Chromatin immunoprecipitation (ChIP) is a powerful tool to study protein-DNA interaction and is widely used in many fields to study proteins associated with chromatin, such as histone and its isoforms and transcription factors, across a defined DNA domain. Here, we show the step-by-step methods currently used in our lab to immunoprecipitate the formaldehyde crosslinked chromatin and further analyze the immuprecipitated DNA by semiquantitative PCR.
Publication Year: 2004
Publication Date: 2004-07-23
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 14
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