Title: [Chromatography of serine proteases on chitin and its derivatives].
Abstract: Chitin containing sorbents have been obtained for isolation and purification of serine proteases. Serine proteases from Bacillus subtilis have been purified 4-5 times and commercial preparations of trypsin and chymotrypsin 1.5-2 times by chromatography on nondeproteinized chitin. On the benzylated derivative of nondeproteinized chitin complete separation of trypsin and chymotrypsin has been achieved by chromatography of crude pancreatin. It has been shown that the protein moiety of chitin is important for preferential sorption of serine type proteases.
Publication Year: 1981
Publication Date: 1981-05-01
Language: en
Type: article
Indexed In: ['pubmed']
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Cited By Count: 1
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