Title: G protein-coupled receptors in drug discovery : methods and protocols
Abstract:1. Purification of Stabilized GPCRs for Structural and Biophysical Analyses James C. Errey, Andrew S. Dore, Andrei Zhukov, Fiona H. Marshall, and Robert M. Cooke 2. Purification and Crystallization of...1. Purification of Stabilized GPCRs for Structural and Biophysical Analyses James C. Errey, Andrew S. Dore, Andrei Zhukov, Fiona H. Marshall, and Robert M. Cooke 2. Purification and Crystallization of a Thermostabilized Agonist-Bound Conformation of the Human Adenosine A2A Receptor Christopher G. Tate and Guillaume Lebon 3. 2D Projection Analysis of GPCR Complexes by Negative Stain Electron Microscopy Alys Peisley and Georgios Skiniotis 4. Nuts and Bolts of CF3 and CH3 NMR Towards the Understanding of Conformational Exchange of GPCRs R. Scott Prosser and Tae Hun Kim 5. Single-Molecule Fluorescence Microscopy for the Analysis of Fast Receptor Dynamics Julia Wagner, Titiwat Sungkaworn, Katrin G. Heinze, Martin J. Lohse, and Davide Calebiro 6. Quantitative Multi-Color Detection Strategies for Bioorthogonally-Labeled GPCRs Minyoung Park, He Tian, Saranga Naganathan, Thomas P. Sakmar, and Thomas Huber 7. Approaches to Characterize and Quantify Oligomerization of GPCRs Sara Marsango, Maria Jose Varela Liste, and Graeme Milligan 8. Monitoring G Protein Activation in Cells with BRET Ikuo Masuho, Kirill A. Martemyanov, and Nevin A. Lambert 9. Use of Fluorescence Indicators in Receptor Ligands Kaleeckal G. Harikumar and Laurence J. Miller 10. Detection and Quantification of Intracellular Signalling Using FRET-Based Biosensors and High Content Imaging Michelle L. Halls, Daniel P. Poole, Andrew M. Ellisdon, Cameron J. Nowell, and Meritxell Canals 11. The Measurement of Receptor Signaling Bias Terry Kenakin 12. Approaches to Assess Functional Selectivity in GPCRs: Evaluating G Protein Signaling in an Endogenous Environment Laura M. Bohn, Lei Zhou, and Jo-Hao Ho 13. Bioluminescence Resonance Energy Transfer Approaches to Discover Bias in GPCR Signaling Elizabeth K.M. Johnstone and Kevin D.G. Pfleger 14. Virus-Mediated Expression of DREADDs for In Vivo Metabolic Studies Mario Rossi, Zhenzhong Cui, Ken-ichiro Nakajima, Jianxin Hu, Lu Zhu, and Jurgen Wess 15. High-Throughput Screening for Allosteric Modulators of GPCRs Robert L. Bertekap, Jr., Neil T. Burford, Zhuyin Li, and Andrew Alt 16. Radioligand Binding Assay for an Exon 11-Associated Mu Opioid Receptor Target Gina F. Marrone, Susruta Majumdar, and Gavril W. Pasternak 17. Docking and Virtual Screening Strategies for GPCR Drug Discovery Thijs Beuming, Bart Lenselink, Daniele Pala, Fiona McRobb, Matt Repasky, and Woody Sherman 18. The Dynamic Process of Drug-GPCR Binding at either Orthosteric or Allosteric Sites Evaluated by Metadynamics Sebastian Schneider, Davide Provasi, and Marta Filizola 19. Experiment-Guided Molecular Modeling of Protein-Protein Complexes Involving GPCRs Irina Kufareva, Tracy M. Handel, and Ruben Abagyan 20. Interaction Fingerprints and Their Applications to Identify Hot Spots Andres F. Marmolejo, Jose L. Medina-Franco, Marc Giulianotti, and Karina Martinez-MayorgaRead More
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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Cited By Count: 2
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