Title: Liquid Chromatography/Mass Spectrometry for the Analysis of Protein Digests
Abstract: Proteolytic digestion, for example with trypsin, in combination with reversed-phase high-pressure liquid chromatographic (HPLC) separation/electrospray ionization (ESI) mass spectrometric detection has become an essential tool in protein analysis. This technique, known as peptide mapping, separates and provides mol-wt information on the peptides resulting from digestion of the protein. In addition, using the instrumentation described in this chapter, sequence information also may be made available (see Sections 3.3.2. and 3.3.5.). Thus, peptide mapping is a highly effective approach to inter alia characterization of protein primary structure and the elucidation of sites of posttranslational modifications. It is the purpose of this chapter to describe the experimental details regarding the chromatographic systems most effectively employed with mass spectrometric detection of peptides, as well as details concerning the mass spectrometer tuning, data acquisition, and data interpretation.
Publication Year: 2003
Publication Date: 2003-11-14
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 9
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