Title: [The effects of intermittent hydromechanics on the differentiation and function of bone marrow stromal derived-osteoblasts in porous calcium phosphate ceramics].
Abstract: To observe the effects of the intermittent hydromechanical stimulation on the differentiation and function of the bone marrow stromal derived-osteoblasts in the porous calcium phosphate ceramics.Rat bone marrow stromal derived-osteoblasts were seeded into porous calcium phosphate ceramic scaffolds at cells density 1 x 10(6) cells/cm(3). The cells-ceramics contrusts were cultured under rotary condition for 1 hour at 4 hours interval. After 4, 7 and 14 days cultivation, the osteoblastic phenotype markers (ALP Activity, Type I Collagen, Osteocalcin, Osteopontin, Osteonectin, and Bone Sialoprotein mRNA expression levels) were analyzed by biochemistry measurement and quantitative RT-PCR technique. Static cell culture as control.Under rotary cell culture condition, the ALP activities and expressions of Type I collagen mRNA increased markedly and reached the peak levels at 7 days. The expressions of other four markers mRNA occurred at 4 days and reached the peak levels at 7 days, then the expressions were down-regulated at 14 days. Under static cell culture as control, the ALP activities and expressions of Type I collagen mRNA increased gradually and reached the peak levels at 14 days. The expressions of other four markers mRNA occurred at 7 days and reached the peak levels at 14 days.The intermittent hydromechanical stimulation could promote the bone marrow stromal derived-osteoblasts differentiation and function which cultured in the porous calcium phosphate ceramics in vitro.
Publication Year: 2002
Publication Date: 2002-05-25
Language: en
Type: article
Indexed In: ['pubmed']
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Cited By Count: 3
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