Title: Measurement of Pancreatic and Salivary Alpha-Amylase in Serum: Comparison of Methods with Five Different Substrates
Abstract: Pancreatic and salivary α-amylase in human serum have been determined with the inhibitor method with 5 different substrates. An end concentration of 60 inhibitor units per liter reagent is sufficient for inhibition of the salivary α-amylase. The recovery of known amounts of pancreatic and salivary α-amylase was excellent. A mean ± SD of 53.1 ± 3.8% of pancreatic α-amylase as percentage of total α-amylase in serum has been observed for the 5 different substrates used. Moreover, it seems that standards obtained from crude saliva or duodenal fluid can be used as well as pure enzymes obtained from a commercial supplier.
Publication Year: 1985
Publication Date: 1985-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 1
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