Title: Bacillus subtilis 의 Ura- 와 Trp- 균주의 육성과 Protoplast 조제 및 Regeneration
Abstract: To investigate the fusion of bacterial protoplasts, two auxotrophic mutants of Bacillus subtilis were isolated after treatment with nitrosoguanidine. Two auxotrophs were uracil-auxotroph and tryptophan-auxotroph. Back mutation frequencies of ura- and trp- -auxotrophs were 2.4×10^(-8) and less than 1.0×10^(-8) respectively. The optimal pH and temperatnre of protoplast formation with lysozyme were 6.5 and 30℃. The optimal lysozyme concentration was 200㎍/㎖. The regeneration frequency of protoplast was 3.3%.
Publication Year: 1985
Publication Date: 1985-01-01
Language: en
Type: article
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