Title: An effect of nerve growth factor on neurite outgrowth (nerve cell culture/ciliary ganglion neurons/conditioned medium/insi
Abstract: Addition of nerve growth factor (NGF) to dis- sociated parasympathetic ciliary ganglion neurons resulted, within 60 min of its addition, in a 2-fold increase in average neurite length and an accompanying enlargement and spreading of neu- ronal growth cones. These effects occurred over a concentration range of NGF of 0.1-10 ng/ml and were blocked by affinity-pu- rified antibody to NGF. Epidermal growth factor, fibroblast growth factor, and angiotensin did not have these effects, al- though insulin at high concentrations was able to induce a re- sponse similar to that of NGF. Dissociated sympathetic chain neurons also responded to NGF with increased neurite lengths, and, in addition, NGF considerably extended the survival time of these neurons in culture. However, the effect of NGF on cil- iary ganglion neurons was limited to neurite outgrowth, and NGF did not promote the survival of these parasympathetic neurons. Nerve growth factor (NGF) promotes neurite outgrowth, neu- ronal survival, and other physiological changes in peripheral sympathetic and sensory neurons and a number of neuron-like clonal cell lines (1-6). However, there has been no evidence of a response of peripheral parasympathetic neurons to NGF. We present evidence for such a response in the present report. This evidence is based on an assay system we have recently devel- oped (7) in which dissociated parasympathetic ciliary ganglion neurons are cultured in the presence of a substratum-condi- tioning factor from heart cell conditioned medium (8-10). In this system the rate of neurite outgrowth is sensitive to the ad- dition of specific agents present in conditioned medium (7) and rat hippocampal extracts (11), and, as shown here, it is sensitive to NGF.
Publication Year: 2016
Publication Date: 2016-01-01
Language: en
Type: article
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