Title: Relationships between base-exchange reaction and synaptosomal phospholipid pool in the rabbit brain in vitro.
Abstract: The calcium-stimulated incorporation of ethanolamine, L-serine and choline into rabbit synaptosomal phospholipids in vitro has been investigated. The synaptosomal membranes were prelabelled in vitro in their choline-, ethanolamine- or serine-phosphoglycerides by base-exchange and then chasing experiments were performed by displacing the lipid-bound base by ethanolamine, choline of L-serine labelled with a different isotope. The results indicate that membrane phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine are substrates for the exchange with all the three mentioned bases. A very small phospholipid pool (0.5-2% of the total available pool) is active in the calcium-dependent exchange between membrane phosphatidylcholine or phosphatidylethanolamine and free bases, whereas the pool of exchanging phosphatidylserine molecules is sensibly larger (2-9%). The possible physiological importance of these small phospholipid pools, involved in base-exchange reaction at brain membrane level, is discussed.
Publication Year: 1977
Publication Date: 1977-05-01
Language: en
Type: article
Indexed In: ['pubmed']
Access and Citation
Cited By Count: 5
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